摘要
以不同的浸种、消毒等方法处理优质番茄百利种子,对其子叶进行培养研究。结果表明:温水浸泡及切口处理种子能获得优质的番茄种子芽苗;以子叶为外植体,在MS+6-BA 2.0 mg/L+IAA 0.5 mg/L培养基上能较好地诱导愈伤组织:愈伤组织在MS+6-BA 1.0 mg/L+IBA0.03 mg/L培养基上分化出芽最多,成苗状况较好;在MS、MS+NAA0.1 mg/L培养基上生根培养无明显差别。
Seeds of tomato Baili were treated with different methods of seed soaking and disinfection. Its cotyledon was cultured and studied. Result showed that good quality tomato sprout was obtained by dipping in warm water and cutting. Callus was induced on MS＋6-BA 2.0 mg/L＋ IAA 0.5 mg/L taking cotyledon as explants. The optimum medium for bud differentiation was MS＋6-BA 1.0 mg/L＋IBA 0.03 mg/L. The seedling had good status. There were no significant differences between the＇rooting mediums of MS and MS＋NAA 0.1 mg/L.
出处
《安徽农业科学》
CAS
北大核心
2007年第29期9211-9212,共2页
Journal of Anhui Agricultural Sciences
关键词
番茄
离体培养
组培苗
Lycopersicon esculentum Miller
In vitro culture
Tissue culture seedlings
