葶苈子水提液对动物实验性心室重构的影响

Effect of aqueous extract from Semen Lepidii seu Descurainiae on ventricular remodeling in experimental animals

目的研究葶苈子水提液对异丙肾上腺素(ISO)诱发小鼠,L-甲状腺素(L-Thy)诱发大鼠实验性心室重构的影响。方法小鼠每天scISO2mg/kg,连续7d;大鼠每天ipL-Thy0.3mg/kg,连续10d,制备心室重构动物模型。各组小鼠每天ig葶苈子水提液6、12g/kg和美托洛尔0.06g/kg,连续7d后,检测心脏指数、血浆环磷酸腺苷(cAMP)、心肌血管紧张素(Ang)量的变化;各组大鼠每天ig葶苈子水提液4、8g/kg和卡托普利0.02g/kg10d后,检测心脏指数、血浆中醛固酮(ALD)及左心肌Ang、羟脯氨酸(Hyp)量的变化。结果模型小鼠的心脏指数、cAMP、Ang量均明显升高,给予美托洛尔0.06g/kg或葶苈子水提液12g/kg均可抑制小鼠左心室重构和肥厚,降低心脏指数和血清cAMP水平及心肌Ang的量(P<0.05)。模型大鼠也出现心肌纤维化,心脏指数、心肌Ang、血浆ALD、心肌Hyp量均明显升高,给予卡托普利0.02g/kg或葶苈子水提液4、8g/kg均可降低大鼠心脏指数和Ang、ALD及心肌Hyp水平(P<0.05)。结论葶苈子水提液具有抑制实验动物心肌肥大、心室重构的作用,其作用机制可能与抑制交感神经系统兴奋性及抑制Ang、ALD等神经内分泌因子激活有关。

Objective To evaluate the effects of aqueous extract from Semen Lepidii seu Descurainiae （SLD） on myocardial hypertrophy induced by isoprenaline （ISO） and L-thyroxine （L-Thy） in experimental mice and rats. Methods The myocardial hypertrophy models of mice were prepared by daily sc ISO 2 mg/kg for 7 d and of rats by daily ip L-Thy 0.3 mg/kg for 10 d. The mice were given aqueous extract from SLD 6 and 12 g/kg or Metoprolol 0.06 g/kg by ig administration once a day for 7 d, then the changes of cardiac indexes, plasma concentration of cAMP, and angiontensin Ⅱ （Ang Ⅱ ） content in myocardium were measured. The rats were given the aqueous extract from SLD 4 and 8 g/kg or Captopril 0.02 g/kg once a day for 10 d, then the changes of cardiac indexes, plasma concentration of ALD, Ang content in left ventricle, and hydroxproline （Hyp） level were measured. Results Compared with the normal mice, cardiac indexs, plasma concentration of cAMP, and Ang Ⅱ content were remarkably increased in the model group （P〈0. 05）, and the cardiac indexes, plasma concentration of cAMP, and Ang Ⅱ content in myocardium were decreased significantly by Metoprolol 0.06 g/kg or the aqueous extract from SLD 12 g/kg （P〈0. 05）, so as to inhibit the left ventricular remodeling and myocardial hypertrophy. Compared with the normal rats, the myocardial fibrosis appeared and the cardiac indexes, plasma concentration of ALD, Ang Ⅱ content, and Hyp level were remarkably increased in the model rats （P〈 0. 05）. The cardiac indexes, plasma concentration of ALD, Ang Ⅱ content, and Hyp level of rats were decreased by Captopril 0. 02 g/kg or the aqueous extract from SLD 4 and 8 g/kg （P〈0.05）. Conclusion The aqueous extract from SLD can attenuate the myocardial hypertrophy induced by ISO and L-Thy, and inhibit the ventricular remodeling in experimental animals. The mechanisms may be related to the inhibition of the excitability in sympathomimetic system and the activation of the neuroendocrine factor of Ang Ⅱ and ALD as well.