乙型肝炎免疫复合物检测方法的建立和优化

Establishment and optimization of detection of the hepatitis B immune complexes

目的建立和优化直接检测乙肝免疫复合物（IC）的方法。方法建立补体Clq固相ELISA、鼠抗-HBs固相ELISA、补体消耗试验等三种检测乙肝IC的方法，并对试验条件进行优化。结果三种方法均可检测出乙肝IC，都有较高灵敏度和重复性。结论选择鼠抗-HBs固相ELISA作为直接检测乙肝IC的最佳方法。

Objective To establish and optimize methods to detect the immune complexes（IC）of hepatitis B virus directly. Methods A Clq solid phase ELISA, mouse anti-HBs MAb solid phase ELISA and the complement consumption assay were established to detect the IC and these methods were optimized. Results All the three methods were highly sensitive, specific and reproducible. Conclusion Mouse anti-HBs MAb solid phase ELISA is the best way to detect IC directly.