支气管哮喘患儿诱导痰液中白细胞介素-4及γ-干扰素水平的研究

Determination of induced sputum IL-4,IFN-γ levels in children with asthma

目的研究支气管哮喘患儿诱导痰液中白细胞介素（IL）-4及γ-干扰素（IFN-γ）水平变化，探讨其在哮喘发病机制中的作用。方法2004年2月～2006年6月儿科住院哮喘患儿36例，用ELISA法测定诱导痰中急性期及缓解期IL-4及IFN-γ水平。结果哮喘患儿诱导痰IL-4水平急性期明显高于缓解期和正常对照组（P〈0．01）；IFN-γ水平急性期低于缓解期及对照组（P〈0．05）；IL-4／IFN-γ比值急性期高于缓解期及对照组（P〈0．05）。急性期患儿诱导痰IL-4水平重症组明显高于轻症组（P〈0．01），IFN-γ水平重症组明显低于轻症组（P〈0．05），IL-4／IFN-γ比值重症组高于轻症组（P〈0．05）。结论哮喘患儿存在Thl／Th2功能紊乱，IL-4及IFN-γ参与哮喘患儿的免疫状态改变，在哮喘发病机制中发挥一定作用。

Objective To study induced sputum IL-4, IFN-γ levels in children with asthma. Methods The IL-4 and IFN-γ levels of 36 induced sputum samples in the acute episode and remission period of asth- matic children were determined by ELISA method. Results The IL-4 levels in the induced sputum were sig- nificantly higher in the acute episode period as compared with children in remission period and controls （P 〈 0.01）. The IFN-γ levels in the acute episode period were lower than that in remission period and controls （P 〈0.05）. The IL-4/IFN-γ ratios in the acute episode period were higher than that in remission period and the controls （P 〈 0.05）. The IL-4 levels in the induced sputum were significantly higher in severe patients than mild patients （P 〈 0.01）. The IFN-γlevels in severe patients were lower than that in mild patients （P 〈 0.05）. The induced sputum IL-4/IFN-γratios in severe patients were higher than that in mild patients （P〈 0.05）. Conclusion Thl/Th2 dysfunction is common in asthmatic children. IL-4 and IFN-γplay a role in the pathogenesis of asthma.