不同产地岩黄连中总生物碱和脱氢卡维丁的比较研究

Study of Total Alkaloids and Dehydrocavidine in Corydalis Saxicola Bunting from Different Areas

目的建立岩黄连中总生物碱和脱氢卡维丁的含量测定方法,并探讨不同产地岩黄连二者含量。方法以脱氢卡维丁为对照,分光光度法测定总生物碱含量;RP-HPLC法测定脱氢卡维丁含量,以Lichrosopher-C18柱为分析柱,0.01mo1/LKH2PO4水溶液(用磷酸调pH=3)-乙腈(75∶25)为流动相,检测波长347 nm,柱温30℃,流速1.0 ml/min。结果广西和贵州产的岩黄连药材总生物碱和脱氢卡维丁含量较高,且广西产的药材两者含量较稳定。结论所建立的方法操作简便快速,结果准确可靠。测定的不同产地样品中,以广西产的药材质量较好且稳定。

Objectlve To establish a method for the content determination of total alkaloids and dehydrocavidine in Corydalis saxicola Bunting , and analyze the two contents from different areas. Methods Dehydreeavidine as the standard and using ultraviolet speetrophotometer to determine the content of total alkaloids. HPLC was performed on the Lichrospher - C18 column ; 0.01 mol/L KH2 PO4 （pH = 3 ） - CH3 CN （75：25 ） served as mobile phase and the detection wavelength was at 283 nm, the column temperature at 30℃ and the flow rate being 1.0 ml/min, to determine the content of dehydroeavidine. Results The contents of total alkaloids and dehydrocavidine from Guangxi and Guizhou were higher, and these two contents from Guangxi were more stable. Conclusion This method is simple, sensitive, specific and can be used for the quality control of Corydalis saxicola Bunting, and the quality from Guangxi is better and stable.