热性惊厥患儿血淋巴细胞亚群变化的临床意义

Changes of Peripheral Blood Lymphocyte Subsets in Febrile Seizure and Its Clinical Significance

目的探讨热性惊厥(FS)患儿外周血淋巴细胞亚群的变化及其临床意义。方法应用流式细胞仪对单纯性FS38例(SFS组)、复杂性FS32例(CFS组)、29例急性上呼吸道感染(上感组)和32例健康儿童(健康对照组)血淋巴细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+、CD1+6CD56+、CD19+进行测定,并进行队列对照研究。结果SFS组血CD3+、CD4+、CD8+、CD4+/CD8+值、CD1+6CD56+、CD19+水平分别为(58.62±13.7)%、(24.54±5.39)%、(29.6±11.39)%、(0.91±0.25)、(12.24±6)%、(18.85±8.22)%;CFS组分别为(55.92±13.43)%、(21.16±5.16)%、(34.24±15.7)%、(0.72±0.26)、(11.94±5.87)%、(21.03±7.85)%;上感组分别为(58.23±12.98)%、(26.72±6.09)%、(23.84±10.56)%、(1.47±0.96)、(14.37±7.39)%、(17.97±8.14)%;健康对照组分别为(60.87±9.59)%、(28.07±6.42)%、(23.62±8.83)%、(1.43±0.85)、(11.94±5.87)%、(21.03±7.85)%。与健康对照、上感组比较,FS组存在CD4+降低,CD8+升高、CD4+/CD8+比值降低,均有统计学意义(Pa<0.05,0.01,0.001);CFS组CD4+、CD4+/CD8+比值较SFS组低,CD8+高,均有统计学意义(P<0.05,0.01)。结论FS患儿体液和细胞免疫均被激活;CFS患儿免疫功能紊乱较SFS更严重。

Objective To explore the changes of peripheral blood lymphocyte subsets in febrile seizure （FS） and its clinical significance. Methods There were total 99 children in this groups of patients versus controls study,38 cases with simple febrile seizure （SFS） ,32 cases with complex febrile seizure （CFS） ,29 cases with acute upper respiratory infections,and 32 healthy children were selected as control group. Flow cy- tometer（FSM） was used to determine the vein blood lymphocyte subsets of marked cell membrane antigens including CD3^＋ , CD4^＋ , CD8^＋ , CD4^＋/CD8^＋ ,CD16^＋ CD56^＋ and CD19^＋ ,then data were analyzed with analysis of variance（ANVOA） by SPSS 12.0 for windows. Results Levels of lymphocyte subsets with CD3^＋ ,CD4^＋ ,CD8^＋ ,CD4^＋/CD8^＋,CD16^＋ CD56^＋ , CD19^＋ in SFS group were（58.62± 13.7）%, （24.54±5.39）%,（29.6±11.39）%,（0.91±0.25）,（12.24±6.0）%,（18.85±8.22）% respectively, and（55.92 ±13.43）%,（21. 16± 5.16 ） %, （ 34.24 ±15.7 ） %, （ 0.72 ± 0.26）, （ 11.94 ± 5.87 ） %, （21.03± 7.85 ） % in CFS group, the group that with the acute upper respiratory infections were （58.23± 12.98 ） %, （26.72 ± 6.09） %, （23.84 ± 10.56 ） %, （ 1.47 ± 0.96 ）, （ 14.37 ±7.39 ） %, （ 17.97 ±8.14 ） %, and （60.87 ± 9.59） %, （28.07 ± 6.42） %, （23.62 ±8.83 ） %, （ 1.43 ± 0. 85 ）, （ 11.94 ±5.87 ） %, （ 21.03 ±7.85 ） % in control group. Both of FS groups showed lower CD4^＋ ,higher CD8^＋ and lower ratio of CD4^＋/CD8^＋ compared with controls, and all had statistics significance （ Pa 〈 0.05,0.01,0. 001 ）. CFS group showed lower CD^4＋ and ratio of CD4^＋/CD8^＋ compared with SFS group, but CD8^＋ showed higher（P 〈 0.05, 0.01 ）. Conclusions Patients with FS manifest have lower CD4^＋ , higher CD8^＋ and lower ratio of CD4^＋/CD8^＋ , indicating that humoral and cellular immunity mechanism being activated. The immunological function disorder in CFS group is more severe than that in SFS group.