食管癌患者血清 P53 蛋白免疫酶联测定

Determination of P53 protein in blood serum from esophageal cancer patient with Elisa

采用免疫酶联法（Ｅｌｉｓａ）测定河南省食管癌高发区林州市居民食管癌患者（ｎ＝２０）和正常人（ｎ＝１１）血清中Ｐ５３蛋白（突变型加野生型）的水平。结果：正常人血清Ｐ５３蛋白水平为（０．１５±０．０９）μｇ／Ｌ，癌患者为（０．２３±０．０４）μｇ／Ｌ。癌患者血清Ｐ５３蛋白水平明显高于正常人（Ｐ＜０．０５）。结果提示：血清Ｐ５３蛋白水平可能是食管癌的一种重要的检测指标。

The P53 protein (wild and mutant type) level in the blood serum was determined with Elisa method on subjects with esophageal cancer (n=20) and the subjects with normal esophageal epithelium (n=11) in the high incidence area of esophageal cancer. Results: the P53 protein level in the blood serum was (0.15±0.09) μg/L in the subjects with normal esophageal epithelium and (0.23±0.04) μg/L in the subjects with esophageal cancer. The P53 protein level in subjects with esophageal cancer was higher than that in the subjects with normal epithelium (P< 0.05). The present results indicated that P53 protein level in the blood serum was an important biomarker for esophageal cancer.