响应面法优化黄芪黄酮提取工艺的研究

Optimization of alcohol extraction techniques of flavonoids from Radix Astragali using response surface methodology

为确定黄芪中黄酮类成分乙醇回流提取的最佳工艺条件,采用高效液相色谱法对4种黄芪黄酮(毛蕊异黄酮、毛蕊异黄酮苷、芒柄花素和芒柄花苷)的含量进行测定;以黄酮得率为指标,采用响应面法对主要工艺参数进行优化并得到回归模型。方差分析结果表明:回归模型较好地反映了黄芪黄酮得率与浸提时间、浸提温度、乙醇体积分数和液固比的关系;最优工艺条件为,提取温度75℃,提取时间2.5 h,乙醇体积分数88.3%,液固比25 mL/g。此工艺条件下提取黄芪黄酮得率为0.977 mg/g,回归模型的预测值与实测值的相对误差<1%,该回归方程与实际情况拟合较好。

To optimize the alcohol reflux process of flavonoid from Radix Astragali response surface methodology （RSM） was employed to investigate the influence of the four main factors including temperature, time, concentration of ethanol, ratio of solvent to raw materiat on the yietd of flavonoid using High-performance liquid chromatography （HPLC）. Regression coefficient and analysis showed the regression model fitted the actual process well. Based on the results of response surface methodology, the best extraction processing parameter was ascertained as follows., tem- perature 75 ℃, time 2.5 h, concentration of ethanol 88.3 % （v/v）, ratio of solvent to raw material 2.5 mL/g. Yield of that optimized procedure was 0.977 mg/g. The experimental values agreed with those predicted within a t % relative error, thus indicating the suitability of RSM in optimizing the extraction of flavonoid from Radix Astragali.