薄层色谱法跟踪N-萘乙酰基-N’-对氯苯甲酰氨基硫脲的合成及生物活性研究

Synthesis of N-Naphthalene Acetyl-N'-p-Chloro Benzoylamino Thiocarbamide Tracked by Thin-layer Chromatography and Its Biological Activity Study

通过α-萘乙酸和SOC l2在无水苯中反应制得α-萘乙酰氯,然后与硫氰酸钾反应生成α-萘乙酰基异硫氰酸酯,再与对氯苯甲酰肼进行加成反应,生成新型化合物N-萘乙酰基-N’-对氯苯甲酰氨基硫脲。用薄层色谱法跟踪最后一步反应,确定了反应时间。产物结构经1H NMR、IR和元素分析确定。生物活性实验结果表明目标产物对稻子根生长具有促进作用,对大肠杆菌和枯草杆菌无明显抑菌作用。

α-Naphthalene acetyl chloride was prepared firstly by the reaction between α-naphthylacetic acid and SOCl2 in anhydrous benzene, then reacted with potassium thiocyanate to obtain α-naphthalene acetyl isosulfocyanate, as taking an addition reaction with p-chloro benzoyl hydrazine the novel compound N-naphthalene acetyl-N＇ -p-chloro benzoylamino thiocarbamide was finally synthesized. The last reaction step was tracked by thin-layer chromatography and the reaction time was determined. The product was characterized by IR spectrum, 1H NMR and elementary analysis. The experimental resuits of investigating biological activity of the title compound indicated that it had promoting action on growth of paddy root and had no obvious bactefiostasic activity against B. subtiles and E. coli.