摘要
目的探讨建立酒精性脂肪肝(alcoholic fatty liver,AFL)动物模型的方法。方法小鼠用50%乙醇灌胃1次/天,连续15天,观察其肝脏病理改变、血液及肝组织的生化变化。结果模型组小鼠肝脏重量明显升高,与正常组比较有显著性差异;病理表现为广泛的脂肪变性,血清甘油三酯(triglyceride,TG)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)、总胆固醇(total cholesterol,CHOL)升高,与正常组比较有显著差异(P<0.01);肝组织TG、ALT、总胆红素(total bilirubin,TBIL)升高,与正常组比较有显著差异;肝组织的CHOL模型组雄性鼠与对照雄性鼠之间比较有显著性差异,雌性鼠之间比较无差异。结论50%乙醇灌胃建立小鼠AFL模型,与AFL的发病相似。
Objective To establish an animal model of alcoholic fatty liver. Methods Mice were exposed to absolute alcohol continuously for up to 15 days via an intragastric feeding. The pathologic and bichemistry alteration of the liver and the changes of serum lipid were observed. Results The weight of model group was higher than those of normal group. Wide hepatic steatosis was induced in model group. Serum triglyceride (TG), alanine aminotransferase(ALT) and total cholesterol (CHOL) of the model group were higher than those of normal group ( P 〈 0.01 ). Liver triglyceride ( TG), alanine aminotransferase (ALT) and total bilirubin (TBIL) of the model group were higher than those of the normal group ( P 〈 0.01 ). For liver CHOL, male mice between model and normal group,was difference, but femal mice was same. Conclusion AFL model of mice established by feeding the absolute alcohol is similar to the episode of the human AFL.
出处
《宁夏医学杂志》
CAS
2007年第11期971-972,共2页
Ningxia Medical Journal