银杏叶多糖提取、纯化及其含量的测定

Extraction,Purification and Content Determination of Polysaccharide in Gingko Leaves

目的研究银杏叶多糖的提取纯化及含量测定。方法通过对不同的提取次数、固液比及提取时间等实验,以及对粗多糖纯化的研究,确立各因素条件,采用苯酚-硫酸法测定其多糖含量。结果各因素条件为浸提温度80℃,提取时间3h,固液比1∶30,提取2次,用活性炭纯化。由此得银杏叶中粗多糖的得率为12.2%,纯化后的多糖得率为9.8%。测定提取的粗多糖中多糖含量为35.7%,纯化后的多糖含量为43.3%,多糖含量测定相对标准偏差小于3.0%。结论该方法纯化效果好,测定重现性好,得出银杏叶中多糖含量为4.3%。

Objective The extraction and purification methods for polysaccharide in Gingko leaves were studied and the content of polysaccharide in it was determined. Methods The condition of each factor for extraction and pruification from the leaves of Gingko was established through the experiments of extraction times, different ratios of material to extraction solvent and extraction time. The method of phenol - sulfuric acid was applied to determination of the content of the polysaccharide. Results The conditions of the factors were extraction temperature 80℃ ,extraction time 3h ,material to extraction solvent 1：30 ,extraction 2 times and activated carbon for purification. Raw polysaccharide in gingko leaves was 12.2% , the polysaccharide after prurified was 9.8% , the content of polysaccharide in the raw polysaccharide was 35.67% ,and in the pure one was 43.32% ,Conclusion The purification method is efficient and stable. The content of polysaccharide in gingko leaves is 4.3%.