反相高效液相色谱法测定山楂中绿原酸的含量

Determinatin of Chlorogenic Acid in Hawthorn by RP-HPLC

本实验中运用反相高效液相色谱对山楂中的有效成分绿原酸进行了分离,并对其进行了含量测定,建立了反相HPLC测定山楂中绿原酸含量的方法。色谱柱:KromasilC18(250mm×4.6mm,5μm),流动相:乙腈-水-甲酸(9:91:0.1),流速:0.8ml/min,检测波长:327nm,柱温:35℃。加样回收率为98.2%,RSD为0.80%。

In this article, the chemical components of the hawthorn were isolated and the contents of chlorogenic acid in the hawthorn were determined by reversed-phase high-performance liquid chromatography on Kromasil C18 column（5 1μ m,250 × 4.6mm）with UV detector, at the wave length of 327 nm, at 35℃. A mixture of water and formic acid and acetronitrile （CH3CN： H2O：HCOOH=9：91：0.1） were used with a flow rate of 0.8 ml/min. The average recovery and relative standard deviation of this method were 98.2% and 0.80%.