温度敏感葡聚糖凝胶的合成及其细胞相容性研究

In vitro cell-biocompability study and synthesis of temperature-sensitive dextran hydrogel

合成了N-异丙基丙烯酰胺（NIPAAm）与葡聚糖的共聚水凝胶，利用红外光谱表征了单体和凝胶结构，研究了水凝胶在去离子水和细胞培养基中的温度响应性，并对凝胶进行了细胞培养和脱附研究．结果表明，共聚凝胶具有很好的温度敏感性和细胞相容性，可以通过控制温度变化，实现对细胞的无损伤脱附．

A series of hydrogels （poly（NIPAAm-co-GMA-Dex）） were synthesized by the copolymerization of glycidyl methacrylate-derivatized dextran （GMA-Dex） and N-isopropylacrylamide （NIPAAm） in aqueous solution. Their swelling properties, cell culture and dettachment.behaviors were studied. It is found that poly（NIPAAm-co-GMA-Dex） hydrogels were temperature-sensitive in deionized water and DMEM （10%FBS）, and they exhibited good biocompatibility. L929 cells could detach from surface of poly（NIPAAm-co-GMA-Dex） hydrogels by controlling temperature.