儿童重症肌无力胸腺提取液免疫抑制活性的研究

Study on Immunosuppressive Activity of Thymus Extract of Children with Myasthemia Gravis

目的:利用胸腺提取液(TE)探讨胸腺在重症肌无力(MG)发病中的作用。方法:取MG 儿童手术摘除胸腺制备 TE,用 MTT 法测定其对胸腺细胞及 PBMC 增殖的影响,用 ELISA 法测定 TE 中 SIL-2R 含量。结果:MG 患者 TE 对 PHA 活化的正常人的胸腺细胞增殖的抑制作用显著增强;对正常人 PBMC 增殖的影响,表现为高浓度抑制、低浓度促进的双向调节作用;TE中 SIL-2R 含量与正常人 TE 无显著差异。结论:MG 患者 TE 有显著异常,与 MG 发病有关,其免疫抑制活性增强的原因,似与 SIL-2R 无关。

Objective:Thymus extract(TE)was used to investigate the role of thymus in patients with myasthenia gravis(MG).Methods:TE was prapared from thymus of the children with MG.Effects of TE on the proliferation of thymocyte and peripheral blood mononuclear celI(PBMC)were evaluated with MTr as- say.Soluble interlukin-2 receptors(SIL-2R)in TE were evaluated with ELISA.Results:The inhibition of TEMG on proliferation of PHA-actived thymocyte of normal human was obviously increased.Effects of TEMG on the proliferation of normal human PBMC were inhibited at high concentration and promoted at low concentration.SIL-2R of TEMG was no obvious difference from that of normal human TE.Conclusion: TEMG possessed obvious abnormality.The abnormality was associated with etiology of MG,but its promo- tion of immunosuppressive activity seemed not associated with SIL-2R.