羧甲基壳聚糖在大鼠颅内组织相容性的初步观察

Studies on Intracraniai Biocompatibility of Carboxymethyl Chitosan in Rats

目的:探讨生物可降解缓释材料羧甲基壳聚糖(CMCS)在大鼠脑组织中的生物相容性,为开发其临床新用途奠定基础。方法:32只SD大鼠随机分为实验组和对照组,分别植入同面积的CMCS和明胶海绵。观察术后大鼠的行为改变和第3,7,14,30天的局部组织反应(HE染色)。结果:两组大鼠在实验期内均无明显行为改变,术后第3,7,14,30天的组织学观察表明CMCS为异物反应,与明胶海绵类似,无明显差异(P<0.05)。随时间推移,CMCS有不同程度降解。结论:CMCS具有良好的脑组织生物相容性,并可安全的降解,可作为颅内植入化疗缓释材料进一步开发。

Objective： To study the intracranial biocompatibility of a biodegradable nature polymer, i.e. carboxymethyl chitosan when used in the rat brain in order to broaden its clinical applications. Method： Thirty-two aduh Sprague Dawley rats were assigned to two groups and unilateral temporal lobe implantation of earboxymethyl chitosan （experiment group）or gel-sponge （control group） were underwent respectively. Local reaction of surrounding tissues of the transplants were observed with H. E stain after the transplantation on 3,7,14 and 30 days respectively. Result： None of the animals showed any behavioral changes or neurological deficits indicating either systemic or localized toxicity from carboxymethyl chitosan and gel-sponge. Compared with gel-sponge, carboxymethyl chitosan evoked a similar localized inflammatory reaction. Conclusion： Carboxymethyl chitosan has been shown in this study to be nontoxic and biocompatible in rat brain.