严重肝病患者并发感染性休克外周血T淋巴细胞亚群的分析

The analysis of peripheral blood T lymphocyte subsets in patients with severe hepatitis and infectious shock

目的探讨并发感染性休克的肝病患者外周血T淋巴细胞亚群的变化。方法在42例并发感染性休克的慢性乙型肝炎患者,取外周血,采用特异性荧光抗体标记、BD流式细胞仪检测其T淋巴细胞亚群。结果慢性乙型肝炎并发感染性休克患者T淋巴细胞亚群表现为CD3+、CD4+、CD8+T淋巴细胞及CD4+/CD8+T比值明显下降(P<0.01),与无并发症组比较,CD3+、CD4+T淋巴细胞均明显下降(P<0.05),但CD8+T淋巴细胞无明显变化(P>0.05),导致CD4+/CD8+比值明显降低(P<0.05)。结论慢性乙型肝炎并发感染性休克患者T淋巴细胞亚群比无并发症组更紊乱,免疫功能更低下。

Objective To analysis the changes of peripheral blood T lymphocyte subsets in patients with chronic hepatitis B and infectious shock. Methods The T lymphocyte subsets were counted by flow cytometry with specific fluo- rescent antibody in 42 patients with hepatitis B and infectious shock. Results The counts of CD3^＋ ,CD4^＋ and CD8^＋ T lymphocyte subsets and the ratio of CD4^＋ CD8^＋in patients with infectious shock dropped significantly （P〈0.05）, and the CD3^＋ and CD4^＋ cells with significantly lower than those in patients without shock, but the CD8 ^＋ cells did not significantly decrease（P〈0.05）, while the ratio of CD4^＋ / CD8 ^＋significantly decreased （P〈 0.05）. Conclusion The cellular immunity of patients with hepatitis B and infectious shock is obviously damaged.