胃舒散对乙醇诱导大鼠急性胃损伤的保护作用

Protective effect of Weishusan on ethanol-induced acute gastric mucosal lesions in rats

目的研究胃舒散对乙醇所致急性胃黏膜损伤(AGML)的保护作用。方法采用乙醇灌胃诱导大鼠AGML模型,采用光镜、扫描电镜和透射观察不同剂量胃舒散(3.0、1.5、0.75 g/kg)对黏膜组织形态学的保护作用,并同时检测胃黏膜局部血流量(GMBF)、跨膜电位(PD)、胃组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和血浆NO水平,等容积的生理盐水和丽珠得乐(1.0 g/kg)分别作为正常对照和治疗对照组。结果胃舒散组胃黏膜损伤指数及组织学评分均较模型对照组显著降低(P<0.05,P<0.01);GMBF及PD较模型对照组显著增高(P<0.01,P<0.05);胃舒散可明显提高胃组织SOD活性(P<0.05)及血浆NO水平(P<0.01)。结论胃舒散对乙醇所致AGML有明显的保护作用,其作用机制可能与增加胃黏膜血流及抗氧化作用有关。

Objective To study the protective effect of Weishusan on acute gastric mucosal lesions induced by ethanol in rats. Methods Wistar rats was instilled intragastrically 1 ml of either Weishusan （3.0,1.5,0.75 g/kg body wt） or a control solution（normal saline） and 1 h later 1 ml of 100% ethanol. Mucosal morphologic changes were assessed by macroscopic examination, quantitative histology, scanning electron microscopy （SEM） and transmission electron microscopy （TEM） 1 h after ethanol instillation. Gastric mucosal blood flow （GMBF）, gastric potential difference （PD）, superoxide dismutase （SOD） activities, malondialdehyde （MDA） contents in gastric tissues and nitric oxide （NO） levels in serum were also measured. Results Gastric mucosal lesion index and pathological scores in Weishusan-treatment group were obviously reduced as compared with the control group （P 〈0.05 ,P 〈0.01 ）. GMBF and PD in Weishusan - treatment group were markedly increased as compared with the control group （P 〈0.01, P 〈0. 05）; Weishusan increased SOD activities （P 〈0.05）in gastric tissues and NO contents in serum（ P 〈0.01 ）. Conclusion Oxygen free radicals and NO may play important roles in ethanol-induced gastric lesions. Weishusan can inhibit gastric lesions and the mechanisms might be related to increase GMBF and antioxidation.