中草药对家蚕病原细菌的体外抑菌试验

TESTS OF CHINESE HERBAL MEDICINES AGAINST SILKWORM PATHOGENIC BACTERIA IN VITRO

本文选用11味中草药对蚕业生产上常见的细菌病病原（黑胸败血菌、苏云金杆菌和灵菌）进行了体外抑菌试验。结果表明：黄岑、黄连、黄柏、大黄、秦皮、连翘对3种病原菌均有不同程度的抑制作用,金银花、蒲公英、苍耳子、黄芪则对3种病原菌无抑制作用。采用平皿内试物液稀释法测定4种单味中草药及其两两组合后的复方中草药对3种病原菌的体外M IC,结果单味中草药对3种病原菌的M IC在31.25-125 mg/mL之间;9种两味药物组合的M IC在7.8125-62.5 mg/mL之间,表现出协同作用和相加作用,显示出中药配伍的优势。

Antibacterial effects of eleven kinds of Chinese herbal medicine in vitro were investigated to some pathogenic bacteria popular in sericulture production.Results showed that there were different degree bacteriostotic actions to Bacillus bombysepticus,Bacillus thuringiensis and Bacillus prodigicsus with any of skullcap,coptis,corktree bark,rhubarb,ash bark and forsythia fruit.But there were no bacteriostatic actions to three bacteria with honeysuckle flower,dandelion,cocklebur fruit and milkvetch.With dilution method of medicine solution in petri dish,the minimal inhibitory concentrations（MIC） of four species of Chinese mono-herbal medicine,nine combinations of each two species of medicine against three bacteria were determined.The results indicated that the MIC of two kinds of Chinese herbal medicine were 31.25～125 mg/mL and 7.8125～62.5 mg/mL.It was showed that the compound formula preparations had advantage in pharmacological activity.