烧冲复合伤早期肺组织ET-1及ETAR转录表达的研究

A study of transcription and expression of endothelin1 and endothelin A receptor in lung at the early stage after combined burnblast injury

目的：探讨内皮素－１（ＥＴ－１）、内皮素Ａ型受体（ＥＴＡＲ）及肿瘤坏死因子α（ＴＮＦα）在烧冲复合伤病理生理过程中的作用和地位。方法：应用放射免疫及原位杂交和斑点杂交等核酸分子生物学技术，观测大鼠烧伤和烧冲复合伤后肺组织ＥＴ－１、ＥＴＲ及ＴＮＦα的转录表达变化、相互关系及与组织细胞损伤的关系。结果：伤后早期肺组织ＴＮＦα及ＥＴ－１的ｍＲＮＡ转录及其产生分泌均显著增加，血浆一氧化氮（ＮＯ）也有所增加，肺组织中的内皮素Ａ型受体（ＥＴＡＲ）ｍＲＮＡ升高，但ＥＴＲ却“下调”，各项指标变化均以烧冲复合伤组为显著，并与肺组织病理变化相关。结论：ＥＴ－１及其受体ＥＴＡＲ等在烧冲复合伤后肺的病理生理过程中可能起着重要作用。

Objective: The role of endothelin1 (ET1), endothelin receptor (ETR) and tumor necrosis factor (TNF) in pathophysiology of the lung after combined burnblast injury was explored. Methods: The changes of transcription and expression of ET1, ETR and TNF( in the lung and their relationship with the tissue damage were studied with in situ hybridization, dot hybridization and radioimmunoassay. Results: The transcription and expression of TNF and ET1 and the level of nitric oxide (NO) in the plasma were significantly increased at the early stage after injury. mRNA of endothelin A receptor was significantly increased while ETR was downregulated. The changes were more significant in the group of combined burnblast injury than in other groups and consistent with the pathological changes of the lung. Conclusion: ET1 and its receptors may play important roles in the pathophysiological process of the lung after combined burnblast injury.