佐剂性关节炎大鼠成纤维样滑膜细胞的体外培养与鉴定

Culture and identification of fibroblast-like synoviocytes from rat with adjuvant arthritis in vitro

目的:建立佐剂性关节炎(AA)大鼠成纤维样滑膜细胞(FLS)的体外培养及鉴定方法,为进一步的实验研究奠定基础。方法:制备AA大鼠模型,用组织块培养法培养AA大鼠滑膜细胞,并通过形态学和流式细胞仪(FCM)进行鉴定。结果:培养的滑膜细胞具有成纤维细胞的形态和特征,呈长梭形极向生长,FCM检测VCAM-1表达率94%以上。结论:成功地培养出了AA大鼠滑膜细胞,可作为进一步实验研究的靶细胞。

Objective： To establish the methods of culture and identification of fibroblast-like synoviocytes （FLS） in rats with adjuvant arthritis （AA） in vitro, and provide a base for further study. Methods： The synovial cells from AA rats were cultured with synovial tissue and identified by morphology and flow cytometer （FCM）. Results： The cultured synovial cells were in a fibrocyte-like form with long fusiform shape and grew in the same direction. The purity of VCAM-1 synoviocytes was over 94 % by FCM. Conclusion： The synovial cells from AA rats were successfully cultured and can be used as target cells for the succedent experimental research.