一氧化氮对家兔冠状动脉阻塞后血小板活化的影响

The inhibitory effect of nitric oxide on platelet activation in a rabbit model of coronary artery occlusion

将30只兔分为3组。Ⅰ组行左冠状动脉前降支(LAD)假扎,Ⅱ组及Ⅲ组行LAD丝线缝扎30min后再灌注4h。Ⅲ组于LAD结扎后静滴硝酸甘油,Ⅰ、Ⅱ组静滴生理盐水。3组均观察手术前后各时点血浆一氧化氮(NO)、血小板表面α颗粒膜蛋白(GMP—140)、血栓素B_2(TXB_2)浓度。结果显示。Ⅰ组手术前后血浆NO、GMP—140、TXB_2浓度无变化,Ⅱ、Ⅲ组在心肌缺血及再灌注后NO浓度降低、GMP—140和TXB_2浓度升高,Ⅱ组上述变化更为显著。Ⅲ组应用类NO物质—硝酸甘油后,再灌注60min时血浆NO浓度较Ⅱ组明显升高,GMP-140和TXB_2明显降低,再灌注4h后上述指标已回复至正常。各时点上NO与GMP—140和TXB_2浓度变化有显著的负相关性。说明NO对活体心肌缺血—再灌注后的血小板活化可能有抑制作用。

30 open-chest rabbits were randomly divided into 3 groups. In group Ⅰ the left anterior descending coronary artery (LAD) was false ligated. Rabbits of group Ⅱ and Ⅲ underwent 30 minuts of LAD occlusion and 4h of reperfusion. After LAD ligated in group Ⅲ were intravenously administered nitroglycerin and in group Ⅰ and Ⅱ were sodium chloride. The level of plasme nitric oxide (NO), alpha-granule membrance protein (GMP-140) on the surface of platelet and thromboxane B_2 (TXB_2) were determined in some time points before and after operation. The results showed that the level of NO, GMP-140 and TXB_2 in group Ⅰ were not difference before and after operation. In group Ⅱ and Ⅲ the level of NO was significantly decreased and the level of the GMP-140 and TXB_2 were significantly increased after myocardial ischemia and reperfusion. Changes in group Ⅱ were more marked than that in group Ⅲ. There were close relation between the value of NO and the value of GMP- 140 and TXB_2. It suggested that NO inhibited platelet activation after myocardial ischemia-reperfusion in vivo.