结扎兔基底动脉后脑干缺血范围的研究

Study of Ischemic Coverage of Brainstem after Ligating Basilar Artery in Rabbit

目的：观察结扎兔基底动脉后脑干的缺血范围。方法：在基底动脉起始部结扎制作脑干局部缺血动物模型，应用墨汁加明胶灌注并显示血管，光镜下分析延髓血管密度变化以确定缺血范围和程度，用尼氏染色观察神经元形态学变化。结果：结扎基底动脉后，脑干血管密度减少（P〈0．05），范围为闩后0．2mm～闩前10mm，包括延髓上部和脑桥；尼氏染色显示缺血区一些神经元胞体肿胀，着色变浅，尼氏体减少，其灰度值明显高于对照组（P〈0．05）。结论：结扎基底动脉起始部可以引起延髓上部和脑桥的局灶性缺血性损伤。

Objective：To observe ischemic coverage of brain stem after ligating basilar artery trunk in rabbits.Methods. Brainstem ischemic model were made by ligating the beginings of the basilar artery trunk, the blood vessels were perfused and showed with prepared Chinese ink and gelatin, the changes in the density of blood vessels were investigated for defining coverage and degree of ischemia. The morphology of neurons in brainstem were defined with Nissl stain. Results ： The density of blood vessels notably decreased in brainstem from 10 mm rostral to the obex to 0.2 mm posterior to the obex including pons and part of superior medulla. The soma was swollen and the Nissl bodies decreased in some of neurons in the ischemic region of brainstem. Conclusion： ligating the basilar artery trunk might induce pons and part of superior medulla focal ischemic damage.