一株植物乳杆菌内化于小鼠回肠派伊尔结及其免疫调节作用的研究

Internalization of Lactobacillus plantarum into mouse ileal Peyer's patches and its immune modulating effects

目的探讨决定Lactobacillus plantarum Lp6在小鼠小肠派伊尔结(PP)内化的细菌源因素,并分析该菌株的免疫调节作用。方法FITC标记不同处理的细菌,分析在含PPs的回肠结扎段内化的情况。给小鼠灌胃活或灭活细菌,研究其对小鼠脾、PP细胞增殖和腹腔巨噬细胞吞噬活性的影响。结果甘露糖可以强烈的抑制细菌内化,灭活和四甲基脲处理可以较明显的减低细菌内化。活菌和灭活菌可以特异性的调节腹腔巨噬细胞、PP细胞和脾淋巴细胞的活性。结论细菌表面甘露糖糖凝集素是影响细菌PP内化的最主要因素。细菌活力、表面疏水性也有一定的作用。活菌和灭活可以不同程度的增强小鼠腹腔巨噬细胞的吞噬活性并抑制脾和PP淋巴细胞增殖。

Objective To investigate the bacterial factors involved in internalization of Lactobacillus plantanum Lp6 into mouse ileal Peyer＇s patches （PPs） and its immune modulating effects. Methods FITC-labeled bacteria and intestinal loops were used to study internalization of treated bacteria into PPs, Viable and inactivated bacteria were administration orally for two weeks for immune response study. Results The internalization could be inhibited by D-mannose, while inactivation and tetramethylurea-treatment could also decrease the internalization of bacteria. Viable and inactivated bacteria could differently modulate the function of mouse immune cells, Conclusion Mannose-specific lectins are the most important factors for determining internalization of the bacteria into mouse ileal PPs, while bacteria viability and its surface hydrophobicity are the minor factors. Bacteria could enhance phagocytic capacity of mouse peritoneal macrophages, but inhibit the proliferation of PP and splenic cells.