电针丰隆穴对高脂血症大鼠一氧化氮和内皮素的影响

Effect of electro-acupuncture at Fenglong(GV 16) on nitric oxide and endothelin in rats with hyperlipidemia

目的:探讨电针(electro-acupuncture,EA)丰隆穴对高脂血症(hyperlipidemia,HLP)大鼠血脂、一氧化氮(nitric oxide,NO)及内皮素(endothelin,ET)含量的影响。方法:将80只Wistar大鼠随机分为4组:正常对照组(饲喂基础饲料)、模型组(饲喂高脂饲料)、电针治疗组(饲喂高脂饲料+电针丰隆治疗)、普伐他汀治疗组(饲喂高脂饲料+普伐他汀钠灌胃)。各组20只。30d后称取各组大鼠体质量,以改良大鼠心脏穿刺采血方法采血,检测血脂、NO以及ET含量。结果:与正常对照组比较,模型组大鼠体质量、总胆固醇(total cholesterol,TC)、甘油三酯(triacylglycerol,TG)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)和ET水平显著升高(P<0.05,P<0.01);高密度脂蛋白胆固醇(high density lipoprotein-cholesterol,HDL-C)、NO水平显著降低(P<0.05)。与模型组比较,普伐他汀治疗组、电针治疗组大鼠体质量、TC、TG、LDL-C水平明显降低(P<0.01),NO水平显著升高。普伐他汀治疗组HDL-C水平显著升高,而电针治疗组升高不明显,两组之间差异有统计学意义(P<0.01)。普伐他汀治疗组ET水平降低(P<0.05),电针治疗组ET水平降低不明显(P>0.05)。结论:电针治疗和西药治疗均对HLP大鼠血脂具有良性调节作用,且能在一定程度上纠正高脂状态下NO和ET水平的失衡。西药对HLP大鼠HDL-C有较好的良性调节作用,而电针对HLP大鼠HDL-C的调节作用有限。

Objective： To investigate the effect of electro-acupuncture （EA） at Fenglong （GV 16） on body weight, blood lipids, nitric oxide （NO） and endothelin （ET） in rats with hyperlipidemia （HLP）. Metheds： Eighty Wistar rats were randomly divided into normal control group （fed normal diet）, untreated group （fed a high-fat diet）, EA-treated group （fed a high-fat diet plus EA therapy） and pravastatin-treated group （feda high-fat diet pluspravastatin tablet）. There were 20 rats in each group. The body weight and the blood content of total cholesterol （TC）, triacylglycerol （TG）, high density lipoprotein-cholesterol （HDL- C）, low density lipoprotein-cholesterol （LDL-C）, NO the and ET of the rats in different groups were measured before experiment and after 30-day treatment. A modified method of cardiac puncture for blood sampling was used for blood collection. Results： Compared with the normal control group, the body weight and the levels of TC, TG, LDL-C and ET in the untreated group were significantly elevated （P〈0.01, P〈0.05）, while the levels of HDL-C and NO were obviously decreased （P〈0.05）. The body weight and the levels of TC, TG, LDL-C demonstrated significant reduction in pravastatin-treated group and EA-treated group as compared with the untreated group （P〈0.01）, and the NO content in pravastatin-treated group and EA-treated group was higher than that in the untreated group （P〈0.01）. Compared with the untreated group, HDL-C level was elevated significantly in pravastatin-treated group, while HDL-C level in EA-treated group was not changed significantly, and there was significant difference in the HDL-C level between pravastatin-treated group and EA-treated group （ P〈 0.01）. The level of ET was decreased obviously in pravastatin-treated group （P〈0.05）, while the level of ET in EA-treated group was not changed significantly （P〈0.05）. Conclusions： Both EA therapy and pravastatin have efficient regulation of body weight and the content of TC, TG, LDL-C and NO in HLP rats. To some extent, they are able to regulate the imbalance between ET and NO content under the condition of HLP. Western medicine such as pravastatin can regulate the HDL-C level in HLP rats, while the effect of EA therapy on regulation of the HDL-C level is limited.