高原肺心病患者血液中HMGB1检测及与痰液中相关因子的关系研究

Detection of HMGB1 in the serum and relationship with some factors in sputum of patients with chronic cor pulmonale at high altitude area

目的探讨高原慢性肺心病患者血液中高迁移率族蛋白1(high mobility group box1,HMGB1)变化及与痰液中相关因子的关系。方法选择慢性肺心病急性期患者48例,健康查体自愿者18人作为对照组。在肺心病患者急性期及治疗病情缓解后1周,检测外周血HMGB1表达及痰液中HMGB1表达、TNF-α水平、SOD活性、MDA水平变化。结果肺心病组急性期患者外周血HMGB1阳性率显著高于缓解期,缓解期显著高于对照组,与痰液HMGB1表达正相关。血液HMGB1表达阳性组痰液TNF-α水平及MDA水平显著高于阴性组。结论HMGB1有助于反映高原慢性肺源性心脏病支气管局部炎症反应。

Objective To detect the expression of HMGB1 in the serum and sputum of the patients with chronic cor pulmonale at high altitude area. Methods Forty-eight patients with chronic cor pulmonale at high altitude area and 18 healthy cases were classified into 2 groups： the experimental group and the control group. The patients in the experiment group were detected in acute stage and remittent stage after 1 week treament. The expression of HMGB1 in the serum and the expression or level of HMGB1, MDA,TNF-α and the activity of SOD in the sputum were detected in both groups. Results The positive rate of HMGB1 was higher in the experimental group than that in the control group. The positive rate of HMGB1 of patients in acute stage was higher than that in remittent stage. The expression of HMGB1 in the serum was positively correlated with the expression of HMGB1 and the level of MDA,TNF-α in the sputum. Conclusion HMGB1 can be one of the impartant indexes in diagnosis,treatment and prognostic assessment in the patients with chronic cor pulmonale at high altitude area.