芍药甘草效应组分血清指纹图谱与药效的相关性研究

Correlation between drug effects and serum HPLC fingerprint of active compositions of Radix paeoniae alba and Radix et Rhizowa Glycyrrhizae

目的:研究芍药甘草效应组分血清指纹图谱与药效的相关性。方法:通过正交实验法,对芍药苷、甘草黄酮、甘草酸不同配伍组合的效应组分进行小鼠扭体的镇痛药理实验;并对效应组分口服后体内血清样品进行HPLC分析,获得各样品的指纹图谱;对血清指纹图谱各色谱峰面积与镇痛药理实验进行相关性分析。结果:各色谱峰面积与镇痛的相关系数不同。结论:为探讨复方中药的药效物质基础提供了有意义的尝试。

AIM： To study the correlation between the drug effects and serum HPLC fingerprint from active compositions of Radix paeoniae alba and Radix et Rhizowa Glycyrrhizae. METHODS： Orthogonal design of L9 （34 ） of the different combinations of three active compositions of paeoniflorin, glycyrrhiza flavoues and Glycyrrhizic acid were prepared, antalgic pharmacological experiments of mice were carried out. The fingerprints of the serum were tested by HPLC method and different chromatographic peaks were obtained. Correlation analysis was applied to interelationship between the area of each peaks and the pharmacologic data. RESULTS ： Correlation coefficients were different between pear areas and analgetic coffec. CONCLUSION ： This study provides a significant try of investigating the compatibility of composite herbal medicines.