HPLC测定木香分气丸中5种成分的含量

Determination of five components in Muxiang Fenqi Pill by HPLC

目的:建立同时测定木香分气丸(丁香、木香、厚朴)中5种成分含量的方法。方法:采用HPLC法同时测定木香分气丸中的5种主要成分:丁香酚、木香烃内酯、去氢木香烃内酯、厚朴酚与和厚朴酚的含量,以Kromasil C18(250 mm×4.6mm,5.0μm)色谱柱为分析柱,以乙腈-甲醇-水(50∶8∶42)为流动相;检测波长210 nm。结果:本色谱条件下丁香酚、木香烃内酯、去氢木香烃内酯、厚朴酚与和厚朴酚之间有良好的分离度,5种成分的浓度与各自峰面积之间线性关系良好,精密度、重复性及加样回收率的相对标准误差均小于1.5%。结论:该法可同时测定木香分气丸中的5种成分,可用于木香分气丸的质量控制。

AIM： To establish a method of simultaneously determining 5 components in Muxiang Fenqi Pill （ Flos Caryophylli, Radix Aucklandiae, Cortex Magnoliae Officinalis ）. METHODS ： Five components ： eugenol, costunolide, dehydrocostuslactone, magnolol and honokiol in Muxiang Fenqi Pill were determined simultaneously by HPLC,using a Kromasil C18 column（250 mm × 4.6 mm,5.0 μm）, acetonitrile-menthanol-water （50： 8： 42） as a mobile phase. The detection wavelength was at 210 nm. RESULTS： The relationship between the concentrations and the peak areas of eugenol, costunolide, dehydrocostuslactone, magnolol and honokiol were linear respectively. The RSD of precision,repeatability and recovery were all less than 1.5%. CONCLUSION： The method is simultaneous determination for five components in Muxiang Fenqi Pill, and can be applied to the quality control of Muxiang Fenqi Pill.