RP-HPLC测定丹芎方中丹参素、阿魏酸、隐丹参酮和丹参酮ⅡA的含量

Simultaneous determination of danshensu,ferulic acid,cryptotanshinone and tanshinone ⅡA in Danxiong Prescription by HPLC

目的：建立丹芎方（丹参，川芎）中多种有效成分同时定量的RP-HPLC方法。方法：应用高效液相色谱法测定丹芎方中有效成分丹参素、阿魏酸、隐丹参酮和丹参酮ⅡA的浓度。采用Agilent ZORBAX SB-C18（5μm，150mm×4．6mm）色谱柱，以甲醇-0．5％（v／v）冰醋酸溶液为流动相进行梯度洗脱，流速为1mL／min，柱温为室温。丹参素、阿魏酸、隐丹参酮和丹参酮ⅡA的检测波长分别为281，320，254和254nm。结果：丹参素、阿魏酸、隐丹参酮和丹参酮ⅡA在线性范围内回归方程的相关系数均大于0．99992，线性关系良好，4种组分检测精密度RSD〈5％。加样回收率为98．1％～104．05％。结论：通过对不同批次及同一批次不同时间的样品的含量进行测定，说明本方法切实可行，稳定可靠，且重现性好，可作为丹芎制剂的质量控制标准。

AIM： To develop a new method for simultaneously determining four effective components in Danxiong Preseription （Radix et Rhizoma Salviae Miltiorrhizae, Rhizoma Chuanxiong ）. METHODS： A RP-HPLC method was set up to determine the concentration of danshensu,ferulie acid, cryptotanshinone and tanshinone ⅡA in Danxiong Prescription. They were successfully separated on an Agilent ZORBAX SB-C18 column（5 μm,150 mm × 4.6 mm） at ambient temperature. A mixture of methanol and 0.5% （v/v） acetic acid in water were used as mobile phase by employing gradient elution at the flow rate of 1.0 mL/min. Danshensu and ferulic acid were detected at 281 and 320 nm,respectively. Cryptotanshinone and tanshinone ⅡA were detected at 254 nm. RESULTS： All the compounds showed good linearity（r 〉0. 999 92） between the peak areas of the componets and their concentration in a relatively wide concentration range. The precision of four components were both below 5%. The recoveries were between 98.1% and 104.05%. CONCLUSION ： The method is simple, sensitive, specificity and good reproducibility. It has been successfully applied to quality evaluation of Danxiong Prescription. It can also be used as a quality control method for Danxiong preparation.