HPLC测定华盖散传统汤剂与颗粒汤剂中盐酸麻黄碱、苦杏仁苷、甘草酸、甘草苷的含量

Determination of ephedrine、laetrile、glycyrrhizic acid and liquiritin in Huagai Powder decoction and granules by HPLC

目的:采用HPLC,测定华盖散(麻黄、苦杏仁、炙甘草等)传统汤剂、颗粒汤剂中盐酸麻黄碱、苦杏仁苷、甘草酸、甘草苷的含量。方法:选用Agilent SB-C18柱,以乙腈-0.1%磷酸(4∶96)为流动相,在207 nm波长测定盐酸麻黄碱;选用Agilent XDB-C18柱,流动相分别以甲醇-水(23∶77),在215 nm波长测定苦杏仁苷;甲醇-0.2 mol/L醋酸铵-冰乙酸(67∶33∶1),在250 nm波长测定甘草酸;乙腈-0.5%冰乙酸(20∶80),在276 nm波长测定甘草苷。结果:方中4个成分在传统汤剂与颗粒汤剂中的含量都有差异,颗粒汤剂中平均含量均大于传统汤剂中平均含量。结论:4种成分的峰都能有效分离,其测定方法简便、准确、重复性良好,方中其它成分对测定无干扰。

AIM： To determine the contents of ephedrine,laetrile, glycyrrhizic acid and liquiritin in Huagai Powder traditional decoction and granule decoction （Herba Ephedrae, Semen Armeniacae Amarum, Radix et Rhizoma Glyeyrrhizae,etc. ） by HPLC. METHODS： An Agilent SB-C18 column was used for ephedrine, the mobile phase was acetonitrile -0.1% phosphoric acid（4： 96）, detection wavelength at 207 nm; An Agilent XDB-C18 column was used for laetrile, the mobile phase was methanol-water （ 23 ： 77 ）, detection wavelength at 215 nm ; An Agilent XDB-C18 column was used for glycyrrhizic acid, the mobile phase was methanol -0.2 mol/L ammonium-acid （67 ：33：1 ） ,detection wavelength at 250 nm; An Agilent XDB-C18 column was＇used for liquiritin,the mobile phase was acetonitrile - 0.5% acetic acid（20： 80） , detection wavelength at 276 nm. RESULTS： There were discrepancies between traditional decoction and granules in the contents of four ingredients, the average contents in granules were more than those in the traditional decoction. CONCLUSION： The peaks of the four ingredients are segregated effectively, and the method is simple, conveient, exact and has good reproducibility,the other ingredients do not have interferences for the determination.