肌苷对缺氧缺血性脑损伤后血管内皮生长因子表达的影响

Effect of inoslne on the expression of VEGF in the cerebral tissue after hypoxia ischemia

目的研究肌苷对缺氧缺血性脑损伤后血管内皮生长因子（VEGF）表达的影响，探讨其神经保护作用机制。方法144只7日龄SD大鼠随机分为三组，即肌苷治疗组，缺氧缺血性脑损伤（HIBD）模型组和正常对照组，前两组参照Rice的方法建立HIBD模型。肌苷组在缺血缺氧后注射100mg/kg肌苷，2次/d，连续7d，HIBD模型组注射等剂量生理盐水，各组分别与缺血缺氧后6h、12h、1d、3d、7d、14d常规灌注取脑免疫组化检测VEGF。结果肌苷组VEGF表达比其他两组明显增多，且于1d、7d出现双峰曲线，14d基本恢复正常水平；HIBD组在缺血缺氧后1d达高峰，然后表达逐渐减少，3d时仅见少量阳性细胞表达，与正常组比较差异无统计学意义（P〉0.05）。结论肌苷可以促进VEGF的表达来发挥缺血缺氧性脑损伤的保护作用。

Objective To observe the expression of vascular endothelial growth factor （VEGF） in the cerebral tissue after hypoxia ischemia in rats and explore the neuroprotective effect of inosine on hypoxic-ischemic encephalopathy, Methods 144 neonatal SD rats were random divided into 3 groups ： HIBD ＋ Inosine group, HIBD group and normal control group. Each group was divided into 6 subgroups, and was injected insosine at the 6h, 12h, 1d, 3d, 7d, 14d after hypoxia ischemia. VEGF in cerebral tissues was determined by the immunohistochemical technique. Results The expression Of VEGF in inosine groups were significantly higher than that in the other two control groups, peaked at the 1d and the 7d, and then decreased rapidly to normal level after 14 days. While the expression of VEGF in HIBD groups peaked at the 24 hour and then decreased rapidly to normal lever after 3 days. Conclusion Inosine could prevent hypoxia ischemia brain damage after hypoxic-ischemic encephalopathy by increasing the expression of VEGF.