动态微波辅助萃取与高效液相色谱联用测定金银花中的绿原酸

Determination of Chlorogenic Acid in Flos Lonicerae Japonicae via Dynamic Microwave-assisted Extraction Coupled On-line with High-performance Liquid Chromatography

通过流动注射的方法将动态微波辅助萃取与高效液相色谱相连接,用于测定金银花中的绿原酸.萃取过程在一个循环体系中完成,萃取后,通过采样环采集到的20μL样品被流动相载带到色谱系统进行分离检测.在最佳实验条件下得到的检出限和测定下限分别为0.25和0.83μg/mL,日内和日间精密度(RSD)分别为3.4%和4.1%,平均加标回收率为98.1%.

A fast method based on dynamic microwave-assisted extraction （DMAE） coupled on-line with highperformance liquid chromatography （HPLC） by means of a flow injection interface has been developed for the determination of chlorogenic acid in Flos Lonicerae japonicae. The extraction was performed in a recirculating system. When a number of extraction cycles were completed, the fractional extract （20 μL） was driven to the analytical column by mobile phase and was measured by a UV detector. The extraction conditions were examined and optimized. The detection and quantification limits of chlorogenic acid obtained at optimized conditions are 0.25 and 0.83 μg/mL, respectively. The intra-day and inter-day precisions （RSD） obtained are 3.4% and 4. 1%, respectively. The mean recovery of chlorogenic acid is 98. 1%. Compared with ultrasonic extraction used in the Chinese pharmacopoeia, the proposed method reduces the amount of solvent, provides more rapid measurement and high extraction yield.