喀什哈尔葡萄组织培养及再生体系的建立

Tissue Culture and Establishment of Regeneration System of Kashihare Grape

以喀什哈尔葡萄单芽茎段为材料,进行了离体组织培养并以实验获得的喀什哈尔葡萄组培苗进行了外植体器官直接再生研究。结果表明:喀什哈尔葡萄单芽茎段最适初始培养基是:MS+6-BA 1.0 mg/L+NAA 0.01 mg/L,侧芽萌芽率达100%;最适继代培养基是:MS+KT 0.1 mg/L+IBA 0.4 mg/L,同时伸长生长与生根生长,生根率达100%。探讨了喀什哈尔葡萄器官再生的影响因素,并以叶片为试材,建立了植株再生体系。叶片不定芽再生的最佳培养基是MS+6-BA 2.0 mg/L+IBA 0.2 mg/L,再生率为14.3%;最佳生根培养基是MS+KT 0.1 mg/L+IBA 0.4 mg/L,生根效果良好,生根率为92%。

In this study, an efficient system of shoot regeneration has been established by use of leaves of Kashihare grape. The results showed that the best auxiliary bud germination medium is MS ＋ 6 - BA 1.0 mg/L ＋ NAA 0.01 mg/L, with the germination rate of 100%. The best subculture medium is MS ＋ KT 0.1 mg/L ＋ IBA 0.4 mg/L. The auxiliary bud germination and the root growth took place at almost the same time in subculture medium, and root rate was up to 100%. The type of the basic medium, the part of explants, the influence of plant growth regulators were investigated in inducing adventitious bud. The results indicated that the highest regenerating rate of adventitious buds were obtained when leaf explants were incubated on MS ＋ 6 - BA 2 mg/L ＋ IBA 0.2 mg/ L ＋ Sucrose 25 g/L. The medium MS ＋ KT 0.1 mg/L ＋ IBA 0.4 mg/L ＋ Sucrose 20 g/L was the best for roots inducing,with an average inducing rate of 92%.