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辐射损伤对小鼠不同组织VEGF表达的影响 被引量:4

The Influence of Iradiate Injury on VEGF Expression in Different Organ of Mice
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摘要 目的探讨小鼠肺、胸腺、小肠、脾不同组织受到辐射损伤后血管内皮细胞生长因子(VEGF)mRNA的表达及意义。方法使用不同剂量(0、4、8 Gy)的X射线照射小鼠,然后取其肺、胸腺、小肠、脾组织。用逆转录多聚酶链反应(RT-PCR)法研究照射后24h不同组织中的VEGF mRNA表达水平。结果经4、8 Gy射线照射后24h,肠、肺组织VEGF mRNA表达水平与正常对照组相比,无显著差异(p>0.05)。脾、胸腺组织VEGF mRNA表达水平与正常对照组相比,均升高。4 Gy射线照射后VEGF mRNA表达水平与其它剂量射线照射组相比显著升高(P<0.01)。结论不同剂量辐射损伤后脾、胸腺组织VEGF mRNA表达增强,肠、肺组织VEGF mRNA表达变化差异不大,VEGF可能对免疫系统比对其他组织的辐射防护作用大,这为放射性损伤的治疗提供了理论依据。 Objective To study the expression and the meaning of the vascular endothelial growth factor(VEGF) mRNA in the lung, thymus, intestines and spleen after suffering from radiative injury.Methods The mice were radiated with different doses of X ray (0,4,8 Gy) and the expression of VEGF mRNA in the lung, thymus, intestines and the spleen after 24 hours' radiation were detected by PT-PCR. Results The expressions of VEGF mRNA in intestines and lung were found to be no significantly difference (P〉0.05) with those in normol control groups after radiated by 4 and 8 Gy ray. The expression of VEGF mRNA in the spleen and thymus gland were higher than those in normol control groups. The expressions of VEGF mRNA in the groups which radiated by 4 Gy were significantly higher than others (P〈0.01). Conclusions The expression level of VEGF mRNA become higher after different radiation dose in the spleen and thymus gland, but have no significant difference in the intestine and lung. Thus VEGF may has stronger radiative protection on immune system than in other organizations, which provides a theoratical basis for treatment of radiative injury.
出处 《实验动物与比较医学》 CAS 2008年第1期27-29,42,共4页 Laboratory Animal and Comparative Medicine
关键词 血管内皮细胞生长因子 辐射损伤 胸腺 小肠 VEGFMRNA VEGF Radiative injury Lung Thymus gland Intestine Spleen VEGF mRNA
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