摘要
目的:研究色谱条件对药品制剂中淫羊藿苷含量测定结果的影响。方法:HPLC,采用C18色谱柱;分别以甲醇-水(54.5∶45.5)、甲醇-水-冰醋酸(54∶46∶0.5)和乙腈-水(25.5∶74.5)为流动相;检测波长为270nm。结果:以甲醇-水(54.5∶45.5)、甲醇-水-冰醋酸(54∶46∶0.5)为流动相时,淫羊藿苷色谱峰和淫羊藿定C色谱峰完全重叠,测得的含量比实际含量高。结论:应对相关国家药品标准进行修订,新建检测方法时,应做好方法学的验证。
Objective：To study The influences of liquid chromatography conditions on the determination of the content of icariin. Method： The HPLC was carried out on C18 column with methanol-water （54. 5： 45.5 ）, methanol-water-acetic acid （54： 46 ： 0. 5 ） or acetonitrile-water（25.5： 74. 5）as a mobile phase respectively. The detective wavelength was 270nm. Results： The peak response for icariin and epimedin C were superimposed completely when methanol-water（-acetic acid） were used as mobile phase, and the determined content of icariin was high compared with the actual content. Conclusion： It is necessary to revise the national standards of the related preparations.
出处
《中国药品标准》
CAS
2008年第1期43-46,共4页
Drug Standards of China
