基质金属蛋白酶及其组织抑制因子在非小细胞肺癌组织中的表达

Analysis of expression of matrix metalloproteinases and tissue inhibitor of metalloproteinases in non small cell lung cancer

目的研究基质金属蛋白酶及组织抑制因子中MMP-2、MMP-9、TIMP．1在不同组织学类型与不同分期人非小细胞肺癌组织中的表达及临床意义。方法应用免疫组化方法检测60例非小细胞肺癌癌组织中MMP-2、MMP-9、TIMP—1的表达状况，并分析其临床病理意义。结果60例肺癌组织中MMP-2、MMP-9、TIMP一1阳性率分别为58．3％、53．3％、53．3％；肺鳞癌组织中TIMP-1的阳性表达率高于肺腺癌（08．2％、21％），MMP-2、MMP-9在肺鳞癌与肺腺癌中阳性率差异无统计学意义；伴淋巴结转移的肺癌组织中MMP-9表达率高于无淋巴结转移者（70．2％、41．2％），而无淋巴结转移的肺癌组织中TIMP-1阳性率（69．2％）高于淋巴结转移者（23．8％）；TIMP-1阳性表达率在Ⅰ、Ⅱ期高于Ⅲ、Ⅳ期（60．8％、28．5％）；存活5年或5年以上者TIMP-1阳性表达率高于存活5年以下者（71．8％、32．1％），MMP-9阳性率在Ⅰ、Ⅱ期与Ⅲ、Ⅳ期差异无统计学意义，存活5年以下者MMP-9阳性表达率则高于存活5年或5年以上者（78．5％、31．2％）。结论TIMP-1与肺癌病理类型、分期及淋巴结转移有关；MMP-9与淋巴结转移有关，与病理类型及分期无关；MMP-9、TIMP-1与肺癌预后相关。

Objective To investigate protein expression status of matrix metalloproteinase and tissue inhibitor of metalloproteinases and their clinical significance in human non small cell lung cancer （NSCLC）, Methods Immunohistochemical method was used to measure the protein expression status of MMP-2, MMP-9, TIMP-1 in 60 cases of NSCLC, and the relationship between the protein expression and elinieopathologieal factors in patients with NSCLC was analyzed. Results MMP-2, MMP-9, TIMP-1 positive rates were 58. 3% ,53. 3% , 53.3% respectively in 60 patients. TIMP-1 positive expression rates in lung squamous cell cancer （68.2%） are statistically higher than that in adenocarcinoma （21% ）, however, there were no statistical differences of MMP-2, MMP-9 positive expression rate between squamous cell cancer and adenocarcinoma. Positive expression rate of MMP-9 （76. 2% ） was higher in patients with lymph node metastasis than those without lymph node involved （41.2%）. Positive expression rate of TIMP-1 was 69. 2% in patients without lymph node metastasis, and it was higher than that in patients with lymph node metastasis （23. 8% ）. TIMP-1 positive rate in stage Ⅰ,Ⅱ （60. 8% ） was higher than that in Ⅲ and Ⅳ（28.5%） , and in those cases surviving 5 years or more than 5 years TIMP-1 also was higher than those surviving less than 5 years. Positive rate of MMP-9 between stages Ⅰ.Ⅱ and Ⅲ,Ⅳ was no statistical difference. Positive rate of MMP-9 in those surviving 5 years or longer was lower than those surviving less than 5 years （31.2% ,78. 5% ）. Conclusions TIMP-1 was related with pathological types, staging and lymph node metastasis of NSCLC. MMP-9 was related with lymph node metastasis and was not related with pathological types and staging. Both MMP-9 and TIMP-1 were associated with the prognosis of NSCLC.