羧甲基壳聚糖的制备及其在黑莓果汁澄清中的应用

Preparation of Carboxymethyl Chitosan and It Application in Clarification of Blackberry Juice

用氯乙酸对壳聚糖进行羧甲基改性,制备不同取代度的羧甲基壳聚糖。将其添加于黑莓果汁,发现黑莓果汁得到澄清。当取代度在0.46～0.86时,黑莓果汁的澄清度随取代度增加而增大;当取代度大于0.86时,澄清度反而降低。比较澄清前后果汁的主要成分,结果表明羧甲基壳聚糖可以减少果汁中蛋白质、多酚和总糖含量。

Carboxymethyl chitosan （N,O-CMC） was prepared by reacting chitosan with chloroacetic acid and various degrees of substitution （DS） were obtained. These N, O-CMC were added into blackberry juice and the results indicated that it could clarify the blackberry juice. The clarification of juice increased with DS values and reached maximum at a DS value of 0.86, and then decreased. The components of clarified juice indicated that CM-chitosan could reduce the contents of proteins, polyphenols and total sugars in blackberry juice.