期刊文献+

茶多酚对酒精性肝病大鼠TNF-α及肝细胞的影响 被引量:6

Effect of tea polyphenols on TNF-αand hepatocytes in rats with alcoholic liver disease
下载PDF
导出
摘要 目的:探讨茶多酚对酒精性肝病大鼠TNF-α表达及肝细胞病变的影响.方法:29只大鼠随机分为:对照组、模型组、茶多酚干预组.白酒ig建模,茶多酚干预.常规切片及特染评价肝组织病变,检测血清AST、ALT、MDA含量,ELISA法检测血清TNF-α、RT-PCR检测肝组织TNF-αmRNA表达.结果:与模型组相比,茶多酚干预组大鼠肝组织学评分(3.00±1.59 vs 4.50±1.31,P<0.05),血清AST、MDA绝对值(87.33±61.00 vs 226.40±81.34,2.75±0.72 vs 7.34±3.06.P<0.05),肝组织TNF-αmRNA表达与TNF-α血清含量均降低(0.55±0.05 vs 0.73±0.07,4.45±0.83 vs 14.33±1.87;P<0.05).结论:茶多酚保护肝细胞线粒体,减轻脂质过氧化,减少TNF-α的合成释放. AIM: To investigate the effect of tea polyphenols on tumor necrisis factor-alpha (TNF-α expression and liver pathological change in rats with alcoholic liver disease. METHODS: Twenty-nine Wistar rats were randomly divided into normal group, model group, and tea polyphenols-intervened group. A model of alcoholic liver disease was induced by gastric perfusion with alcohol and intervened with tea polyphenols. Liver tissue sections were stained with H&E. Serum levels of AST, ALT and MDA were determined. Serum TNF-α was detected by ELISA. RT-PCR was performed to determine TNF-α expression in liver tissue. RESULTS: The scores of pathology (3.00 ± 1.59 vs 4.50± 1.31, P 〈 0.05), serum levels of AST and MDA (87.33 ± 61.00 vs 226.4 ± 81.34, 2.75 ± 0.72 vs 7.34 ± 3.06, P 〈 0.05) as well as TNF-α (0.55 ± 0.05 vs 0.73 ± 0.07, P 〈 0.05) and TNF-α mRNA expression (0.55 ± 0.05 vs 0.73 ± 0.07, P 〈 0.05; 4.45± 0.83 vs 14.33 ± 1.87, P 〈 0.05) were lower in the tea polyphenols-intervened group than in the model group. CONCLUSION: Tea polyphenols can protect hepatocytes from damage, decrease lipid peroxidation and TNF-α release.
出处 《世界华人消化杂志》 CAS 北大核心 2008年第4期356-360,共5页 World Chinese Journal of Digestology
关键词 酒精性肝病 茶多酚 肿瘤坏死因子Α 酶联免疫吸附法 逆转录聚合酶链式反应 Alcoholic liver disease Tea polyphenols Tumor necrosis factor-alpha Enzyme linked immunosorbent assay RT-PCR
  • 引文网络
  • 相关文献

参考文献12

  • 1Suganuma M, Okabe S, Oniyama M, Tada Y, Ito H, Fujiki H. Wide distribution of [3H](-)- epigallocatechin gallate, a cancer preventive tea polyphenol, in mouse tissue. Carcinogenesis 1998; 19: 1771-1 776.
  • 2酒精性肝病诊疗指南[J].肝脏,2006,11(1):66-68. 被引量:43
  • 3刘本英,王平盛.茶多酚生物学活性的研究进展[J].热带农业科技,2006,29(2):28-30. 被引量:16
  • 4Kaviarasan S, Ramamurthy N, Gunasekaran P, Varalakshmi E, Anuradha CV. Epigallocatechin-3- gallate(-)protects Chang liver cells against ethanolinduced cytotoxicity and apoptosis. Basic Clin Pharmacol Toxicol2007; 100:151-156.
  • 5高采平,彭燕.肿瘤坏死因子-α与酒精性肝病[J].国外医学(消化系疾病分册),2005,25(6):379-381. 被引量:9
  • 6Ponnappa BC, Israel Y, Aini M, Zhou F, Russ R, Cao QN, Hu Y, Rubin R. Inhibition of tumor necrosis factor alpha secretion and prevention of liver injury in ethanol-fed rats by antisense oligonucleotides. Biochem Pharmacol2005; 69:569-577.
  • 7Day CP. Treatment of alcoholic liver disease. Liver Transpl 2007; 13:S69-S75.
  • 8张宇,陈韶华,张幸国,任国平,萨小婴,虞朝辉,厉有名.茶多酚治疗慢性酒精性肝损伤的实验研究[J].中华肝脏病杂志,2005,13(2):125-127. 被引量:15
  • 9周晓蓉,龚作炯,袁光金,张频.绿茶多酚对实验性酒精性肝损伤大鼠的治疗作用[J].世界华人消化杂志,2006,14(1):50-56. 被引量:13
  • 10Bode C, Bode JC. Effect of alcohol consumption on the gut. Best Pract Res Clin Gastroenterol 2003; 17: 575-592.

二级参考文献43

  • 1胡国平,刘凯,赵连三.多烯磷脂酰胆碱(易善复)治疗酒精性肝病和脂肪肝的系统评价[J].肝脏,2005,10(1):5-7. 被引量:108
  • 2Varilek GW, Yang F, Lee EY, et al. Green tea polyphenol extract attenuates inflammation in interleukin-2-deficient mice, a model of autoimmunity. J Nutr, 2001, 131: 2034-2039.
  • 3Yang F, Oz HS, Barve S, et al. The green tea polyphenol (-)-epigallocatechin-3-gallate blocks nuclear factor-kappa B activation by inhibiting I kappa B kinase activity in the intestinal epithelial cell line IEC-6. Mol Pharmacol, 2001, 60: 528-533.
  • 4Yin M, Wheeler MD, Kono H, et al. Gastroenterology, 1999;117:942-952.
  • 5Kishore R, Hill JR, McMullen MR, et al. Am J Physiol Gastrointest Liver Physiol, 2002 ; 282 : G6-15.
  • 6Nanji AA, Jokelainen K, Lau GK, et al. J Pharmacol Exp Ther, 2001;299:832-839.
  • 7Zhou Z, Wang L, Song Z, et al. Am J Pathol, 2003;163: 1137-1146.
  • 8Iimuro Y, Gallucci RM, Luster MI, et al. Hepatology, 1997;26:1530-1537.
  • 9Daniluk J, Szuster-Ciesielska A, Drabko J, et al. Alcohol,2004; 33:9-15.
  • 10Pastorino JG, Shulga N, Hoek JB. Am J Physiol Gastrointest Liver Physiol, 2003 ;285 :G503-516.

共引文献89

同被引文献112

引证文献6

二级引证文献31

相关主题

;
使用帮助 返回顶部