摘要
目的:探讨茶多酚对酒精性肝病大鼠TNF-α表达及肝细胞病变的影响.方法:29只大鼠随机分为:对照组、模型组、茶多酚干预组.白酒ig建模,茶多酚干预.常规切片及特染评价肝组织病变,检测血清AST、ALT、MDA含量,ELISA法检测血清TNF-α、RT-PCR检测肝组织TNF-αmRNA表达.结果:与模型组相比,茶多酚干预组大鼠肝组织学评分(3.00±1.59 vs 4.50±1.31,P<0.05),血清AST、MDA绝对值(87.33±61.00 vs 226.40±81.34,2.75±0.72 vs 7.34±3.06.P<0.05),肝组织TNF-αmRNA表达与TNF-α血清含量均降低(0.55±0.05 vs 0.73±0.07,4.45±0.83 vs 14.33±1.87;P<0.05).结论:茶多酚保护肝细胞线粒体,减轻脂质过氧化,减少TNF-α的合成释放.
AIM: To investigate the effect of tea polyphenols on tumor necrisis factor-alpha (TNF-α expression and liver pathological change in rats with alcoholic liver disease.
METHODS: Twenty-nine Wistar rats were randomly divided into normal group, model group, and tea polyphenols-intervened group. A model of alcoholic liver disease was induced by gastric perfusion with alcohol and intervened with tea polyphenols. Liver tissue sections were stained with H&E. Serum levels of AST, ALT and MDA were determined. Serum TNF-α was detected by ELISA. RT-PCR was performed to determine TNF-α expression in liver tissue.
RESULTS: The scores of pathology (3.00 ± 1.59 vs 4.50± 1.31, P 〈 0.05), serum levels of AST and MDA (87.33 ± 61.00 vs 226.4 ± 81.34, 2.75 ± 0.72 vs 7.34 ± 3.06, P 〈 0.05) as well as TNF-α (0.55 ± 0.05 vs 0.73 ± 0.07, P 〈 0.05) and TNF-α mRNA expression (0.55 ± 0.05 vs 0.73 ± 0.07, P 〈 0.05; 4.45± 0.83 vs 14.33 ± 1.87, P 〈 0.05) were lower in the tea polyphenols-intervened group than in the model group.
CONCLUSION: Tea polyphenols can protect hepatocytes from damage, decrease lipid peroxidation and TNF-α release.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第4期356-360,共5页
World Chinese Journal of Digestology