深层培养诸因素对金针菇菌丝体生长的影响

The Effect of Some Factors in Submerged Culture on Mycelial Growth of Flammulina velutipes

本文研究了在500ml摇瓶振荡培养条件下,培养基的初始pH值、不同碳源、不同氮源对金针菇菌丝体生长的影响和培养过程中培养液pH值的变化规律,并测定了菌丝体和子实体的主要营养成分及氨基酸含量。结果表明,深层培养7d,菌丝体生长量最高,培养液的pH值用培养时间的延长而逐渐下降,第7天最低;培养基最适pH为5.5～7.4;金针菇的适用碳源是葡萄糖、蔗糖和纤维素;适用氮源是蛋白胨和酵母膏。菌丝体与子实体的营养成分种类相似,而菌丝体的蛋白质含量和氨基酸总量均高于子实体。

The effect of primary pH, different carbon sources, different nitrogen sources on mycelial growth and the pH changing law of liquid medium during the culture of Flammulina velutipes in 500ml shake flask were studied in this paper. The main nutrients and amino acid content in mycelia and fruitbodies were also analysed. The results showed that after submerged culture for seven days, the mycelial yield was the highest while the pH of the liquid medium decreased with the extending of culture time and fell to the lowest at the seventh day. The optimum pH of the medium was 5.5-7.4. The optimum carbon sources and nitrogen sources for F. velutipes were glucose, sucrose, cellulose, peptone and yeast extract resp.. The nutrient compositions of mycelia and fruitbodies were almost the same, but the content of protein and total amino acids in mycelia was higher than that in fruitbodies.