Sephadex G-10凝胶色谱系统测定头孢美唑钠的聚合物

Determination of the polymer in cefmetazole sodium by sephadex G-10 column

目的 建立凝胶色谱法测定头孢美唑钠的聚合物的方法。方法 色谱柱为葡聚糖凝胶Sephadex G-10（16mm×35cm），流动相A：0.02mg·mL^-1磷酸盐缓冲液（pH7．O）；流动相B：水。流速为1．0mL·min^-1。检测波长为254nm，进样量为200μl。结果 头孢美唑钠在5、O～60.0mg·mL^-1范围内，浓度与聚合物的峰面积呈良好的线性关系（r=0.9996）。结论 该方法简便，准确，灵敏度高，重现性好。

OBJECTIVE To establish a method for determination of the polymers in Cefmetazole Sodium.METHOD The chromatographic column was Sephadex G-10 （16mm× 35cm）, mobile phase A： 0,02 mg· mL^-1 phosphate buffer （pH7.0） ; mobile phase B： pure water; the flow rate was 1,0 mL· min-1, the detection wavelength was 254nm and the injection volume was 200μL. RESULT The calibration curve was linear in the concentration range of 5.0～60.0mg·mL^-1（r=0,9996）.CONCLUSION The method is simple, accurate, sensitive and reproducible. It can be used to determine the polymer in cefmetazole Sodium.