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^(137)Cs γ射线辐照对手工分血小板制品细胞因子灭活的研究 被引量:3

Sterilization by ^(137)Cs Gamma-ray Irradiation on Cytokine Levels in Manually Manipulated Platelet Concentrates
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摘要 目的探讨137Cs γ射线辐照对手工分血小板制品中细胞因子的灭活作用,明确手工分血小板制品辐照处理技术的可行性。方法手工分离血小板后,用25Gy的137Cs γ射线照射,于照射后0、1、3、5d检测血小板制品中IL-β、IL-6、IL-8和TNF-α等细胞因子含量的变化;并分别于贮存的第0、5天分别进行pH值测定和血小板计数。结果对照组和照射组的细胞因子含量均随保存时间延长而显著增加,但对照组中细胞因子含量增加更为明显;而在同一保存时间对照组中细胞因子含量高于照射组(P<0.05);保存过程中,辐照组血小板计数及pH值与对照组差异无统计学意义(P>0.05)。结论137Cs γ射线辐照可以显著降低手工分血小板制品中细胞因子的水平。 OBJECTIVE To study the sterilization by gamma radiation on the cytokine levels in manually manipulated platelet concentrates, and its practicability. METHODS Manually manipulated platelet concentrates were irradiated by gamma ray at 25 Gy. Detected the quantity of cytokine levels after irradiation on dd 0, 1, 3, and 5. Platelet counts and pH value were detected on the d0 and d5. RESULTS The contents of cytokines increased with storage time both in irradiation and control groups. But the contents of cytokines in the control group increased more significantly than in the irradiation one (P〈0.05), No significant differences in platelet counts and pH value between the two groups on d0 and d5 were found. CONCLUSIONS The methods of ^137Cs gamma-ray irradiation make it's role in reducing the cytokines in manually manipulated platelet concentrates.
出处 《中华医院感染学杂志》 CAS CSCD 北大核心 2008年第3期389-391,共3页 Chinese Journal of Nosocomiology
关键词 ^137CS Γ射线 血小板制品 细胞因子 输血 ^137 Cs Gamma-ray Irradiation Platelet concentrates Cytokine Blood transfusion
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