端粒酶活性定量分析的方法学研究

STUDIES ON THE METHODOLOGY FOR QUANTITATIVEDETERMINATION OF TELOMERASE ACTIVITY

本文报道一种端粒酶活性定量测定方法，并对反应体系和条件、分离、测定等多种可能影响测定结果的因素进行了初步探讨。结果表明本方法的批内和批间变异系数分别为１５．６％和１６．４％。放射性计数在７００～７０００的范围内与端粒酶活性呈现良好的线性关系。用本方法测定了部分肿瘤活检组织样品，其结果与文献值基本一致。

In this paper,an assay of quantitative determination of telomerase activity has been studied.And some fators such as reacting system and conditions,methods of isolation and fixation,and so on ,which may influence the reproduction were also investigated.Results showed that the inter-batch and extra-batch variations were 15.6% and 16.4%, respectively.It revealed well linear relationship between telomerase activity and the value within 700￣7000/min determined by radioactive count.The telomerase activity of some specimens of cancer results from the procedures of this assay were accordant with literature.