酒精处理对青春期大鼠海马CA1区NGF和TrkA表达的影响

Effect of alcohol treatment on the expressions of NGF and TrkA in CA1 area of hippocampus of the adolescent rat

为探讨青春期饮酒致学习记忆力下降并长期持续的可能机制,本研究选用30d龄大鼠,给予2%、5%和9%的酒精作为唯一饮料分别喂养3d,动物在停酒后0、3、7和14d处死;对照组动物以自来水代替酒精。用免疫组织化学方法(ABC法)检测海马CA1区神经生长因子(NGF)和TrkA的表达,Motic3.2图像分析系统测定阳性反应产物的平均灰度值。结果显示,TrkA的表达在停酒后0d实验组与对照组相比差异无显著性(P>0.05),3、7和14d均较对照组明显加强(P<0.05);NGF的表达在停酒后0d实验组与对照组相比差异无显著性(P>0.05),3d较对照组明显减弱(P<0.05),7和14d两组与相应对照组相比差异无显著性(P>0.05)。以上结果提示,CA1区NGF表达的相对不足可能是青春期饮酒致学习记忆力下降的原因之一。

To investigate the possible mechanism of adolescent drink leading to a decline of learning and memory ability, and long-term effects, 30 days old rats were deployed in the present experiment and given 2%, 5% and 9% alcohol as the sole beverage to breed for consecutive 3 days, respectively. Then the rats were sacrificed on the 0, 3, 7 and 14 d after stopping drink. The control group rats were given tap water instead of alcohol, The nerve growth factor （NGF） and TrkA expressions in CA1 area of hippocampus were observed by immunohistochemieal technique （ABC method）, and the mean gray values of immunoreactive product were detected by Motic3.2 image analysis system. The results showed that the expressions of NGF and TrkA had no significant differences between experimental groups and control groups on 0 d after stopping drink （ P 〉0.05 ）. The expressions of TrkA on 3, 7 and 14 d after stopping drink were increased significantly compared with control groups （ P 〈 0.05 ）. NGF expression on 3 d after stopping drink was attenuated obviously compared with control groups （ P 〈0.05 ）. There were no differences of NGF expression on 7 and 14 d after stopping drink compared with control groups. These results suggested that the relative insuffieienee of NGF expression in CA1 area of hippocampus may be one of the reasons for the long-term deficits of learning and memery after alcohol treatment in the adolescent.