大黄酚对脑缺血再灌注小鼠学习记忆障碍及耐缺氧的影响

Effects of chrysophanol on learning and memory impairment and anoxia-tolerance in mice with cerebral ischemia reperfusion

目的:探讨大黄酚对脑缺血再灌注小鼠学习记忆障碍的保护作用及其耐缺氧的影响。方法:60只健康小鼠随机分为假手术组、模型对照组、大黄酚高、中、低剂量组,采用暂时性阻断两侧颈总动脉的方法制备小鼠脑缺血再灌注损伤的模型,进行避暗法实验,测试其被动回避能力;另取60只健康小鼠,分组、造模方法同上,进行常压耐缺氧实验,测试其生存时间。结果:大黄酚可明显延长脑缺血再灌注小鼠进入暗室的潜伏期,减少错误次数;明显延长常压耐缺氧的生存时间。结论:大黄酚对脑缺血再灌注小鼠学习记忆功能有保护作用和抗缺氧的作用。

Objective： To study the protective effects of chrysophanol on learing and memory impairment and anoxia-tolerance in mice with cerebral ischemia reperfusion. Methods： Sixty health mice were randomly divided into five groups： sham group, control group, chrysophanol groups （10, 1, 0.1mg/kg）. Cerebral ischemia reperfusion injury was produced in conscious mice by temporarily obstructing bilateral common carotid arteries, Passive avoidance was measured by step-through test; Another sixty health mice, animal groups and models were the same as above, the survival time was measured by normal pressure anoxia tolerance test, Results： Chrysophanol could prolong latent period, decrease error times and prolong the survival time in normal pressure anoxia tolerance mice, Conclusion,, Chrysophanol showed protective effects on learning and memory of mice with cerebral ischemia reperfusion and anoxia-tolerance.