白介素8白介素10及γ-干扰素在肺炎支原体肺炎中的作用

The role of IL-8,IL-10 and IFN-in mycoplasma pneumoniae pneumonia

目的探讨肺炎支原体肺炎(MPP)免疫学发病机制,寻找对病情轻重有重要提示意义的免疫学指标,指导临床治疗。方法选择北京儿童医院呼吸科病房2006年7月至2007年1月确诊的MPP患儿34例,采用双抗体夹心ELISA方法检测急性期和恢复期血清白介素8(IL-8)、IL-10及γ-干扰素(IFN-γ)质量浓度。并以同期行健康体检的儿童20名作为对照组。结果MPP患儿血清IL-8质量浓度急性期和恢复期较对照组明显升高(P<0.05,P<0.01),恢复期较急性期明显升高(P<0.01);血清IFN-γ质量浓度急性期较恢复期和对照组明显升高(P<0.01);血清IL-10质量浓度急性期较恢复期和对照组降低(P<0.05,P<0.01)。急性期重症组血清IFN-γ质量浓度高于轻症组(P<0.05);而IL-10质量浓度低于轻症组(P<0.05)。结论IL-8、IFN-γ参与了MPP的发病过程;MPP急性期存在一过性IL-10降低;急性期血清IL-10、IFN-γ浓度对病情轻重有重要提示作用。

Objective To investigate the contribution of IL-8,IL-10 and IFN-γ to the immunology pathogenesis and to find the significant cytokines to direct the clinical management.Methods The serum levels of IL-8,IL-10 and IFN-γ in 34 patients at acute stage and in 30 patients at convalescence stage and in 20 age-and sex-matched control subjects were determined by the method of ELISA.Results The serum levels of IL-8 in patients at acute stage and at convalescence stage were significantly higher than that of control group（P〈0.05,P〈0.01）,and the level at convalescence stage was significantly higher than those at acute stage（P〈0.01）.The serum level of IFN-γ at acute stage was significantly higher than those at convalescence stage and than that of the control group（P〈0.01）.The serum level of IL-10 at acute stage was Lower than those at convalescence stage and that of control group（P〈0.05,P〈0.01）.The serum level of IFN-γ in severe cases at acute stage was higher than those in mild cases（P〈0.05）,and the level of IL-10 was lower than those in mild cases（P〈0.05）.Conclusion IL-8 and IFN-γ participate in the onset of MPP.The serum level of IL-10 declines temporarily at acute stage.The serum levels of IFN-γ and IL-10 at acute stage play a significant role in indicating the patient＇s conditions.