食管内脏高敏感性动物模型的建立和评价

Establish and evaluation of the rat model of esophageal visceral hypersensitivity

目的建立食管内脏高敏感性动物模型。方法采用腹腔注射鸡卵清蛋白(OVA)基础致敏联合食管酸灌注的方法处理SD大鼠,并采用免疫组织化学方法和显微图像分析技术评价内脏高敏感性-食管化学刺激大鼠模型的可靠性。结果OVA基础致敏联合食管酸灌注组大鼠被激活了一个复杂而广泛的大脑网络,其在额顶皮质、岛叶、扣带皮质、中央杏仁核、Klliker-Fuse核、疑核、臂旁核、下丘脑室旁核、丘脑室旁核、三叉旁核、孤束核、最后区、延髓网状核等Fos样免疫活性(FLI)神经元的数目均显著高于其余各组(P<0.05)。模型组大鼠在中央杏仁核、臂旁核、室旁核、三叉旁核、孤束核的FLI阳性产物的平均光密度(OD)值亦较其余各组明显增高(P<0.05)。结论预先腹腔注射鸡卵清蛋白基础致敏联合食管酸灌注可成功建立食管内脏高敏感性动物模型。

Objective To establish a reliable rat model of esophageal visceral hypersensitivity. Methods Healthy Spragne-Dawley （SD） rats received basal ovalbumin-sensitized combined with intra-esophageal mucosal acid exposure. The neuronal expressions of c-los proto-oncogene were detected with immunohistochemical counter-staining and computerized color image analyzer. Results The basic ovalburain-sensitized plus esophageal acid perfusion in model group initiated a high density expression of c-fos-immunoreactive （Fos-IR） neurons in multineuronal networks. A significantly higher number of Fos positive neurons was found in the model group than those in the corresponding regions of other groups（ P 〈 0.05 ） in the following brain areas ： frontal and parietal cortex, insular cortex, cingnlated cortex, central amygda- loid nucleus,the Kolliker-Fuse nucleus, the nucleus ambignus, parabrachial nucleus, hypothalamic paraventricular nucleus, paraventricular thalamic nucleus, paratrigeminal nucleus, the nucleus of solitary tract, area postrema, reticular nucleus of medulla,The OD values of Fos-IR neurons were also increased in the central amygdala , parabrachial nucleus, paraventricular nucleus, the paratrigeminal nucleus and NTS in the model group than that in the corresponding regions of other groups （ P 〈 0.05 ）. Conclusion The reliable rat model of esophageal visceral hypersensitivity has been established.