云南普米、傈僳、纳西、怒四个少数民族补体C4遗传多态性的检测

INVESTIGATION ON THE GENETIC POLYMORPHISM OF THE FOURTH COMPONENT OF COMPLEMENT (C4) IN THE POUPLATION OF PUMI, LISU, NAXI AND NU IN YUNNAN, CHINA

为了解人类补体成份Ｃ４遗传多态性，用神经氨酸酶和羧肽酶Ｂ处理ＥＤＴＡ抗凝血浆，继以琼脂糖高压电泳、免疫固定及薄层激光密度扫描等技术对云南普米、傈僳、纳西和怒４个少数民族进行补体Ｃ４遗传多态性的检测，并与湖北汉族进行对比。结果显示：５个民族的Ｃ４Ａ／Ｃ４Ｂ表型分布不完全相同。在基因频率方面：Ｃ４Ａ除普米族以Ｃ４Ｑ０最高外，其余均以Ｃ４Ａ３为高；Ｃ４Ｂ在５个民族中都以Ｃ４Ｂ１最高，其余参差不等。湖北汉族与４个少数民族之间的差异主要表现在Ｃ４Ａ２，Ｃ４Ａ３，Ｃ４Ａ５和Ｃ４Ｂ２２；４个少数民族之间同样存在较大差异，普米族与其他３个少数民族的差异最为显著，其次是傈僳族，纳西族和怒族之间的差异不显著。

The genetic polymorphism of the fourth component of complement (C4)in Pumi, Lisu, Naxi and Nu minorites of China was investigated with the two enzyme(NAse+CPseB) method according to Sim and Cross, and Zhang WJ et al. The Han's values were put together for comparison. Five common C4A/C4B phenotypes were obtained and compared among these five nationalities. The frequency of C4A3 was the hightest in the five nationalities except C4Q0 was the highest in Pumi. The frequency of C4B1 was the highest in the five nationalities. Comparison of the allele frequencies of these five nationalities showed that the differences between Han and the four differences among five nationalities were mainly in the allotypes C4A2, C4A3, C4A5 and C4B22. Comparison of the allele frequencies of the four minorities showed that the differences between Pumi and the other three minorities were greater than those between the minorities themselves.