猪流感病毒的分离与鉴定

Isolation and Identification of Type A Swine influenza virus

从广东某猪场表现发热和流鼻涕等呼吸道症状的猪群采集28份样品,用鸡胚分离到9株病毒。将病毒传代至第6代,经血清学鉴定、理化特性检查、电镜观察、生物学特性检查和人工致病试验,结果表明,4株分离株为H1N1亚型猪流感病毒,5株为H3N2亚型猪流感病毒。

Nine virus strains were isolated from 28 clinical samples collected from pigs in Guangdong in 2006. The isolates were propagated by six passages in the allantoic cavities of 9-day-old specific pathogen free chicken embryos, and identified by using serological, physiochemical, morphological, biological tests and experimental infection test. The results indicated that four isolates are H1N1 swine influenza viruses and five isolates are H3N2 swine influenza viruses.