妊娠各期人乳头瘤病毒的感染率及其基因分型

Infection rate and genotyping methods of carrying out human papilloma virus in different period of pregnancy

目的:对不同妊娠期的同一批妇女及非妊娠期健康妇女的宫颈分泌物分别进行HPV检测,以探讨其感染率、基因分型和HPV感染影响因素之间的关系。方法:利用DNA杂交导流技术和基因芯片技术检测不同妊娠期及非妊娠期健康妇女的宫颈标本各100例,并进行基因分型。结果:100例非妊娠期健康妇女的宫颈标本中,HPV感染率为12%(12/100)。检出高危基因型(HPV16/18/3152/56型)8例;低危基因型(HPV6/11型)3例;常见亚型(HPV53)1例。在100例不同妊娠期的标本中,早、中、晚孕期感染率分别为19%(19/100)、20%(20/100)及35%(35/100)。检出高危基因型(HPV16/18/31/33/52/58/68型)26例;低危基因型(HPV6/11/42型)12例;常见亚性(HPV53/66)2例。其中有多重型感染患者。结论:妊娠期HPV感染率明显高于非妊娠期健康妇女,且感染率随妊娠进展而逐渐上升,其中高危亚型尤为明显,需要引起临床重视。

Objective：Carrying out HPV detecting on the secrete of woman cervix between different period of pregnancy and non -pregnancy in order to discuss its infection rate and genotyping. Methods-Making use of the flow - guiding technology of DNA hybridization and the gene chip technology to check I00 specimens in different period of pregnancy and healthy women of non - pregnancy respectively, and carrying out genotyping. Results： In 100 specimens of non - pregnancy： infection rate was 12 %. Eight samples of high dangerous genotypes （ HPVI 6/18/31/52/56 type） were inspected, 3 samples of low dangerous geno- types （HPV6/I I type） inspected, I sample of common subtypes （HPV53） was inspected. In I00 specimens of different period of pregnancy, early, middle, late pregnancy infection rate was 19%, 20% and 35% ,respectively： 26 examples of high dangerous genotypes （ HPVI6/18/31/33/52/58/68 type） were inspected, 12 examples of low dangerous genotypes （ HPV6/11/42 type） inspected, 2 examples of common subtypes （HPV53/66） inspected. Among them, some patients were affected by many mixed types. Conclusion：It is obviously that the infection rate is higher in pregnancy than in non -pregnancy. With the advancement of period of pregnancy, the infection rate for HPV is higher and higher, especially for high dangerous genotypes, which should be paid close attention to.