链脲佐菌素与四氧嘧啶诱导建立兔1型糖尿病模型的比较

Comparation of Inducing Type 1 diabetes models on rabbits byStreptozotocin and Alloxan

目的比较研究以链脲佐菌素与四氧嘧啶作为诱导剂建立兔1型糖尿病模型的效果及特点。方法将不同浓度的链脲佐菌素和四氧嘧啶分别采用耳缘静脉注射和腹腔注射方法对新西兰大白兔进行1型糖尿病诱导,监测给药后试验兔血糖及体重变化并进行葡萄糖耐量试验(glucose tolerance test,GTT)。根据相关结果分析两种诱导剂在不同诱导方式下的建模效果及特点。结果两种药物对糖尿病兔的血糖浓度均具有显著影响(P<0.05),但在相同给药方式下,链脲佐菌素的诱导作用明显弱于四氧嘧啶,且经静脉给药组的血糖浓度明显高于腹腔给药组。葡萄糖耐量试验中各试验组糖尿病兔对葡萄糖刺激的反应不同程度减弱。结论在静脉给药条件下链脲佐菌素是更为合理安全的诱导剂,在腹腔给药条件下则宜采用四氧嘧啶作为诱导剂。

Objective To compare the effect and character of inducing type 1 diabetes models on rabbits.Methods The STZ and ALX of different concerntrations were injected into the rabbits through auricle edge vein and abdominal cavity to induce the type 1 diabetes models.The blood glucose concerntration and body weight of the rabbits were detected and the glucose tolerance tests were performed on the models.The effection and character of inducing type 1 diabetes models on rabbits by the different methods and drugs were analyzed according the datas.Results The blood glucose concerntration of the rabbits were obviously affected by STZ and ALX（P〈0.05）.But the inducing effect of STZ was feebler than that of ALX by the same injection methods and the blood glucose concerntration of the rabbits in intravenous injection group was much higher than that of in peritoneal injection.The glucose stimulating responses in treating groups were attenuated in different degrees in glucose tolerance test.Conclusion STZ could be regarded as the reasonable and safe inducing drug by intravenous injection while the ALX should be used as the inducing drug by intravenous injection.