HIF-1α和TopoⅡ在子宫内膜癌中的表达及意义

EXPRESSIONS OF HIF-1α AND ToPo Ⅱ IN ENDOMETRIAL CARCINOMA TISSUES

目的探讨子宫内膜癌组织中HIF-1α和TopoⅡ的表达。方法采用免疫组织化学法(SP法)检测60例子宫内膜癌、30例增生期子宫内膜组织中HIF-1α和TopoⅡ的表达,并结合临床病理资料进行分析。结果HIF-1α在子宫内膜癌组织中的阳性表达率明显高于增生期子宫内膜(χ2=13.31,P<0.01);HIF-1α的阳性表达随着子宫内膜癌临床分期的升高而明显升高(χ2=8.15,P<0.05);TopoⅡ在子宫内膜癌组织中的阳性表达率明显低于增生期子宫内膜(χ2=8.44,P<0.01);在子宫内膜癌组织中,HIF-1α与TopoⅡ的表达具有关联性(χ2=9.6,P<0.01)。结论HIF-1α在子宫内膜癌发生、发展中起重要的作用;子宫内膜癌中HIF-1α可能通过某种机制参与了TopoⅡ介导的多药耐药。

Objective To study the relation ship of the expressions of HIF-1α and Topo Ⅱ in endometrial carcionma tissues. Methods The expressions of HIF-1α and Topo Ⅱ were examined in 60 cases of endometrial caicinoma and 10 cases of proliferative endometrium by immunohistochemistry staining. Results The positive rate of HIF-1α in endometrial caicinoma tissues was higher than that in normal endometnum（χ^2= 13.31, P 〈0.01）. There was positive correlation between HIF-1α positive expression and clinical stage（χ^2 = 8.15, P 〈 0.05）. The positive rate of Topo Ⅱ in endometrial carcinoma tissues was significantly lower than that in proliferative stage （χ^2 = 8.44, P 〈 0.01 ）. There was positive correlation between HIF-1α and Topo Ⅱ expressions in endometrial carcinoma tissues（χ^2= 9.6, P 〈 0.01）. Conclusion The positive expressions of HIF-1α may have an important role in the development of endometrial carcinoma; HIF-1α might participate in the multidrug resistance mechanism that mediated by Topo Ⅱ in endometrial caicinoma.